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| 猪伪狂犬病PCR检测方法的建立与应用 | |
| 引用本文： | 孙德刚,吴发兴,李晓成,黄保续,王洪斌,张燕霞. 猪伪狂犬病PCR检测方法的建立与应用[J]. 中国动物检疫, 2007, 24(2): 29-30 |
| 作者姓名： | 孙德刚 吴发兴 李晓成 黄保续 王洪斌 张燕霞 |
| 作者单位： | 1. 中国动物卫生与流行病学中心,山东青岛,266032;东北农业大学,黑龙江哈尔滨,150030 2. 中国动物卫生与流行病学中心,山东青岛,266032 3. 东北农业大学,黑龙江哈尔滨,150030 |
| 摘 要： | 根据Genbank中PRV gD基因序列设计引物建立了检测猪场狂犬病的PCR方法并对某省25份猪伪狂犬病疑似病料进行检测，结果在2S份病料中，检出伪狂犬阳性病料7份，阳性率接近30%。通过检测结果及特异性和敏感性实验发现此检测方法敏感度很高。这种检测方法适合科学研究、临床诊断以及流行病学调查。 |
| 关 键 词： | 伪狂犬病病毒 检测 |
| 文章编号： | 1005-944X(2007)02-0029-02 |
Establishment and Application of PCR Method for Detection of Porcine Pseudorabies Virus |
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| Sun DeGang,Wu FaXing,Li XiaoCheng,Huang BaoXu,Wang HongBin,Zhang YanXia. Establishment and Application of PCR Method for Detection of Porcine Pseudorabies Virus[J]. China Animal Health Inspection, 2007, 24(2): 29-30 | |
| Authors: | Sun DeGang Wu FaXing Li XiaoCheng Huang BaoXu Wang HongBin Zhang YanXia |
| Affiliation: | 1.China Animal Health and Epidemiology Center, Qingdao,266032; 2.Northeast Agricultural University,Harbin |
| Abstract: | According to PRV gD gene sequence in Genebank,a pair of primers were designed to develop a PCR and 25 suspicious samples from a province were detected using the developed method. Of 25,7 samples were positive. The positive rate of PRV approached 30%. It was concluded that the PCR method was of high sensitivity, and it could be applicable to research, clinical diagnosis and epidemiological investigation. |
| Keywords: | PCR GC buffer |
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