| 胡杨悬浮细胞原生质体游离及质膜单离子通道测定 |
| |
| 引用本文： | 陈少良,戴松香,李金克,王沙生,Andrea Polle.胡杨悬浮细胞原生质体游离及质膜单离子通道测定[J].中国林学,2002,4(1):1-4. |
| |
| 作者姓名： | 陈少良 戴松香 李金克 王沙生 Andrea Polle |
| |
| 作者单位： | 1. 北京林业大学生物科学与技术学院,北京,100083
2. 哥廷根大学森林植物研究所,哥廷根,37077,德国 |
| |
| 基金项目： | 国家自然科学基金，the Foundation for the Author of National Excellent Doctoral Dissertation of China，北京大学校科研和教改项目 |
| |
| 摘 要： | 该文以胡杨 (Populuseuphratica)悬浮细胞为材料 ,利用 1 0 %纤维素酶“onozuka”R 10、0 0 1%果胶酶Y 2 3、0 15 %离析酶R 10和 0 1%半纤维素酶的混合酶液消化细胞壁 ,得到原生质体 .并利用膜片钳细胞贴附技术分别测到质膜内向和外向单通道电流 .通道电流在不同水平上变化 ,表明并非只有一种类型的通道开放 .树木细胞原生质体的分离以及利用膜片钳测定细胞质膜离子通道的成功实践为深入研究木本植物抗盐的细胞学机制奠定了基础
|
| 关 键 词： | 胡杨 悬浮细胞 原生质体 膜片钳 细胞贴附技术 单通道 |
Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica |
| |
| Chen Shaoliang,Dai Songxiang,Li Jinke,Wang Shasheng,Andrea Polle,Aloys Hüttermann.Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica[J].Forestry Studies in China,2002,4(1):1-4. |
| |
| Authors: | Chen Shaoliang Dai Songxiang Li Jinke Wang Shasheng Andrea Polle Aloys Hüttermann |
| |
| Abstract: | The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1.0 % cellulase "onozuka" R-10, 0.01% pectolyase Y-23,0.15% macerozyme R-10 and 0.1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell-attached recording of patch-clamp technique.In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species. |
| |
| Keywords: | Populus euphratica suspension cells protoplasts patch\|clamp cell\|attached recording technique single ion channel |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
