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| 农杆菌介导Ferritin基因转化苹果的研究 | |
| 引用本文： | 叶霞,黄晓德,陶建敏,乔玉山,姚泉洪,章镇.农杆菌介导Ferritin基因转化苹果的研究[J].果树学报,2005,22(4):387-389. |
| 作者姓名： | 叶霞 黄晓德 陶建敏 乔玉山 姚泉洪 章镇 |
| 作者单位： | 1. 南京农业大学,南京,210095;河南黄河园林绿化工程有限公司,郑州,450003 2. 南京农业大学,南京,210095 3. 上海农业科学院,上海,201106 |
| 基金项目： | 国家自然科学基金项目(30370987)，江苏省应用基础项目(BJ200018)。 |
| 摘 要： | 以皇家嘎拉苹果的叶片为转化受体,通过根癌农杆菌介导将铁结合蛋白(Ferritin)基因导入受体中,诱导获得了抗性芽.经PCR检测从抗Km植株中选择到4株阳性植株,进一步经PCR-Southern杂交证实外源基因已整合到4株苹果基因组中,RT-PCR检测表明,Ferritin基因在4株转基因植株中得到了表达. |
| 关 键 词： | 铁结合蛋白 根癌农杆菌 基因转化 苹果 |
| 文章编号： | 1009-9980(2005)04-387-03 |
| 修稿时间： | 2004年11月30 |
Agrobacterium-mediated transformation of apple with ferritin gene |
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| Ye Xia,HUANG Xiao-de,Tao Jian-min,QIAO Yu-shan,YAO Quan-hong,ZHANG Zhen.Agrobacterium-mediated transformation of apple with ferritin gene[J].Journal of Fruit Science,2005,22(4):387-389. | |
| Authors: | Ye Xia HUANG Xiao-de Tao Jian-min QIAO Yu-shan YAO Quan-hong ZHANG Zhen |
| Institution: | YE Xia1,2,HUANG Xiao-de1,TAO Jian-min1,QIAO Yu-shan1,YAO Quan-hong3,ZHANG Zhen1* |
| Abstract: | The bean iron-binding gene(Ferritin) was introduced into the leaves of Royal Gala apple cultivar by Agrobacterium-mediated genetic transformation,and some Kanamycin-resistant shoots were obtained. Four positive plants were selected from those Kanamycin-resistant plants by PCR amplification,and the results of PCR-Southern blotting analysis confirmed that the foreign gene has been integrated into the genome of Gala apple cultivar,RT-PCR revealed that the ferritin gene was expressed in the four transgenic plants. |
| Keywords: | Ferritin Agrobacterium tumefaciens Transformation Apple |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |