Differential cytotoxicity of the isolated protein fraction of Escumite bean (Phaseolus acutifolius) |
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Authors: | María Teresa González-Garza Virginia Sousa Angela Sotelo |
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Affiliation: | (1) Unidad de Investigación Biomédica del Centro Médico Nacional, Instituto Mexicano del Seguro Social, Apartado Postal 73-032, 03020 México, DF, México |
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Abstract: | The Escumite bean (Phaseolus acutifolius) is an edible legume which in raw form is highly toxic to rats. Proteins were separated by DEAE cellulose and affinity chromatography. Hemagglutinating activity, trypsin inhibitory effect, cytotoxicity on human peripheral lymphocytes and on intestinal epithelial cells of rats, and mitogenic activity were assayed with each protein fraction. Hemagglutinins and trypsin inhibitory fractions showed differential toxicity with lymphocytes as compared to intestinal epithelial cells. A protein fraction without the previous activities was cytotoxic mainly to intestinal epithelial cells. |
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Keywords: | bean lectin trypsin inhibitor cytotoxic |
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