葡萄7个重要花发育相关基因序列特征的分析

根据Genbank上发表的7个葡萄花发育相关基因(VvAG、VvAP3、VvFLC、VvFT、VvSOC1、VvAP1、VvFUL)的序列信息,设计了7对特异引物,并采用PCR方法对‘香悦’葡萄中的同源基因进行了扩增,将获得的各基因片段克隆至pMD18-T载体后转化E.coliDH5α,经PCR鉴定后进行了序列测定。将得到的7个基因的编码框序列及推导出的氨基酸序列与Gen-Bank中公布的相应序列进行同源性比较,并构建系统进化树。从本研究发现不同葡萄品种的花发育基因存在核苷酸与氨基酸序列上的差异,表现在某些碱基发生转换与颠换以及氨基酸类型的变化。分析与多种植物同源基因的进化关系表明,7个葡萄花发育相关基因分别与不同树种的同源基因表现出较高的相似性,在进化上具有一定的随机性。

Characterization of Seven Important Genes Involved in Grapevine Flower Development

According to the nucleotide sequences of seven important genes(VvAG,VvAP3,VvFLC,VvFT,VvSOC1,VvAP1,VvFUL) involved in grapevine flower development deposited in Genebank,seven pairs of primers were designed and used to amplify their homologues in grapevine cultivar 'Xiangyue'.All the PCR products were ligated to pMD18-T vector,and then the recombinant vector was transformed into E.coli DH5α strain.The anticipated clones checked by PCR were cultured and sent for sequencing.The open reading frame sequences of the 7 genes and the deduced amino acid sequences were aligned with their corresponding homologues deposited in Genebank,and seven phylogenetic trees were constructed.The results indicated that the nucleotide and amino acid sequences of the 7 genes in different grapevines were different at some loci and they were more identical with the homologues in different plants,suggesting they evolved in different directions.