| 辣椒胞质雄性不育系（CMS）子叶培养植株再生 |
| |
| 引用本文： | 邓明华,文锦芬,邹学校. 辣椒胞质雄性不育系（CMS）子叶培养植株再生[J]. 农业科学与技术, 2009, 10(1): 39-42 |
| |
| 作者姓名： | 邓明华 文锦芬 邹学校 |
| |
| 作者单位： | 邓明华,DENG Ming-hua(中南大学隆平分院,湖南长沙,410125;云南农业大学园林园艺学院,云南昆明,650201);文锦芬,WEN Jin-fen(昆明理工大学现代农业工程学院,云南昆明,650224);邹学校,ZOU Xue-xiao(中南大学隆平分院,湖南长沙,410125) |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)，云南省自然科学基金 |
| |
| 摘 要： | 笔者以细胞分裂素、生长素、赤霉素、硝酸银为培养基的附加成分，利用辣椒CMS9704A和8214A子叶作为外植体，研究辣椒CMS子叶再生体系，为辣椒CMS的遗传改良奠定基础。
|
| 关 键 词： | 子叶培养 胞质雄性不育系 辣椒 植株再生 细胞分裂素 再生体系 遗传改良 CMS |
In vitro Plant Regeneration of Pepper Cytoplasmic Male Sterility (CMS) Lines via Cotyledon Culture |
| |
| DENG Ming-hua,WEN Jin-fen,ZOU Xue-xiao. In vitro Plant Regeneration of Pepper Cytoplasmic Male Sterility (CMS) Lines via Cotyledon Culture[J]. Agricultural Science ＆ Technology, 2009, 10(1): 39-42 |
| |
| Authors: | DENG Ming-hua WEN Jin-fen ZOU Xue-xiao |
| |
| Affiliation: | 1. Branch of Longping, Central South University, Changsha 410125; 2. College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201 ;3. Faculty of Modern Agricultural Engineering, Kunming University of Science and Technology, Kunming 650224) |
| |
| Abstract: | An in vitro shoot regeneration procedure was developed in pepper (Capsicum annuum L.) cytoplasmic male sterility (CMS) lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzladenine (6-BA), indole-3-acetic acid (IAA), gibberellic acid (GA3) and silver nitrate (AgNO3). From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L AgNO3, for the explants callus and bud cluster, the maximum differentiation rates (respectively 100.0% and 58.3%) and average number of adventitious bud from each explant (respectively 18.8 and 13.2) were obtained. The optimum medium combination for the elongation of adventitious bud was determined to be: MS+ 3.0 mg/L 6-BA + 1.0 mg/L IAA+ 5.0 mg/L AgNO3+ 2.0 mg/L GA3, from which the elongation rates of buds from callus and bud cluster were both 100%, and the average number of per explant adventitious bud number reached 6.3 and 5.8, respectively. And all the elongated shoots were successfully rooted on half-strength MS medium supplemented with 0.3-0.5 mg/L IAA. |
| |
| Keywords: | Capsicum annuum L. CMS line Cotyledon culture Plant regeneration |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
