| 酯酶同工酶染色的优化 |
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| 引用本文： | 王波,张丽敏,谭亮,于洋,曹玉伟,李佳琳,罗志文. 酯酶同工酶染色的优化[J]. 农产品加工．学刊, 2009, 0(6). DOI: 10.3969/j.issn.1671-9646(X).2009.06.030 |
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| 作者姓名： | 王波 张丽敏 谭亮 于洋 曹玉伟 李佳琳 罗志文 |
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| 作者单位： | 佳木斯大学,生命科学学院,黑龙江,佳木斯,154007 |
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| 摘 要： | 利用聚丙烯酰胺凝胶电泳,测定鱼的酯酶同工酶,对已有染色程序进行改良,用KMnO4取代传统的坚牢蓝RR盐,溶于Tris-Gly(pH值8.3)中,达到了理想效果。
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| 关 键 词： | 酯酶同工酶 醋酸萘酯 染色 |
Optimization of Staining Method for Esterase Isozymes |
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| Wang Bo,Zhang Limin,Tan Liang,Yu Yang,Cao Yuwei,Li Jialin,Luo Zhiwen. Optimization of Staining Method for Esterase Isozymes[J]. Nongchanpin Jlagong.Xuekan, 2009, 0(6). DOI: 10.3969/j.issn.1671-9646(X).2009.06.030 |
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| Authors: | Wang Bo Zhang Limin Tan Liang Yu Yang Cao Yuwei Li Jialin Luo Zhiwen |
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| Affiliation: | College of Life Science;Jiamusi University;Jiamusi;Heilongjiang 154007;China |
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| Abstract: | In order to improve staining method,polypropylene-amide erect-board eletrophresis were used to make an analysis of the EST isozyme.KMnO4 instead of the fast blue salt RR and was dissolved in Tris-Gly(pH8.3),the stain result was very well. |
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| Keywords: | esterase isozyme naphthylacetate staining method |
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