云南野生和逸生苜蓿资源POD和EST同工酶分析

为了摸清云南苜蓿(Medicago L.)资源的遗传多样性,采用聚丙烯酰胺凝胶不连续垂直板电泳系统,对30份云南野生和逸生苜蓿材料过氧化物酶(POD)和酯酶(EST)同工酶酶谱进行了分析。结果表明:供试材料的2种同工酶酶带数均为10条,多态位点百分数均达到100%。除二倍体材料外,其余材料均具有多条相同的共有谱带,有着丰富的多态性,但POD和EST酶谱存在明显差异;供试材料的POD和EST遗传距离均在0.00~1.00之间,其中1号、3号和4号二倍体苜蓿,与其他逸生材料遗传距离较大,亲缘关系较远;其余均为紫花苜蓿(M.sativa L.),POD和EST的遗传距离分别在0.00~0.56和0.00~0.33之间。采用类平均法(UPGMA)Nei氏距离进行聚类分析,表明30份供试材料亲缘关系的远近与地理分布呈一定的相关性。这将为云南苜蓿资源遗传关系的研究提供基础数据。

Isozyme Analysis of Peroxidase and Polyphenol Oxidase in Wild and Escaped Alfalfa Germplasm Resources of Yunnan

Peroxidase(POD) and esterase(EST) of 30 wild and escaped alfalfa germplasm resources in Yunnan were analyzed by PAGE to study their genetic diversities.Results showed that both isozymes separated 10 enzyme bands by PAGE with 100% polymorphic loci.Except diploid wild alfalfa resources,the same characteristics of isozyme bands were observed in other materials with rich genetic polymorphism.Enzyme spectrums of POD and EST were significantly different.Genetic distances of POD and EST in all tested materials ranged from 0.00～1.00.There was larger genetic distance between diploid wild alfalfa(1,3 and 4) and escaped alfalfa compared with others.Genetic distances of POD and EST in tested Medicago Sativa L.materials ranged from 0.00～0.56 and 0.00～0.33 respectively.UPGMA cluster analysis of Nei’s distance indicated that the relationships among 30 wild and escaped alfalfa germplasm resources were related to their geographic distribution.These results are useful in studying genetic relations of alfalfa germplasm resources in Yunnan.