Elimination of phytoplasma by stem culture from mulberry plants (Morus alba) with dwarf disease |
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| Authors: | Q. DAI,F.-T. HE,& P.-Y. LIU |
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| Affiliation: | Department of Biology, Shandong University, Jinan 250100; Institute of Plant Protection, CAAS, Beijing 100094; Silk Corporation of Linyi, Linyi Region, Shandong Province, 276001;P.R.China |
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| Abstract: | Stem culture in vitro was attempted for the elimination of mulberry dwarf phytoplasma. Stem segments, from mulberry plants ( Morus alba ) seriously infected with the phytoplasma, were cultured on Murashige and Skoog (MS) solid nutrient medium without phytohormones for 2–3 months. The tissue cultures, and regenerated plants grown under greenhouse conditions for 3 years, were tested by polymerase chain reaction (PCR) and 4,6-diamidino-2-phenylindole (DAPI) staining for the presence of the phytoplasma, and also observed for mulberry dwarf symptoms. From 70% to 90% of regenerated plants were apparently freed from the phytoplasma since they remained PCR-negative, DAPI-negative and symptom-free for as long as tested (3 years). |
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