| 雄性育性基因RNA干扰载体的构建与鉴定(英文) |
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| 引用本文： | 王文锋,王红霞,穆灵敏. 雄性育性基因RNA干扰载体的构建与鉴定(英文)[J]. （《农业科学与技术》）编辑部, 2009, 0(3) |
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| 作者姓名： | 王文锋 王红霞 穆灵敏 |
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| 作者单位： | 新乡医学院生命科学技术系;新乡医学院形态学实验室; |
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| 基金项目： | Supported by National Natural Science Foundation of China(39870532)~~ |
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| 摘 要： | cDNA fragment of fertility gene MS2 from cotton was cloned by RT-PCR approach, it was highly homologous with relevant genes of Brassica napus and Arabidopsis thaliana. According to the principles of constructing RNAi vector, sense and antisense fragments of MS2 gene carrying restriction endonuclease recognition sites were amplified via PCR technique, ligated with the first intron of upland cotton chinase gene, then inserted into artificially modified plant expression vector pBI121, yielding RNAi vector pBGP12MSIn. The results showed that RNAi vector pBGP12MSIn harboring MS2 gene driven by anther specific promoter BGP was successfully constructed. Our results laid a foundation for studying the function of this gene and genetic transformation of plant male sterile lines.
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| 关 键 词： | 感受态细胞 限制性内切酶 反基因 基因片段 反义基因 引物 表达载体 重组质粒 酶切鉴定 大肠杆菌 |
Construction and Identification of Male Sterile RNAi Vector |
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| WANG Wen-feng,WANG Hong-xia,MU Ling-min. Construction and Identification of Male Sterile RNAi Vector[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2009, 0(3) |
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| Authors: | WANG Wen-feng WANG Hong-xia MU Ling-min |
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| Affiliation: | WANG Wen-feng1,WANG Hong-xia1,MU Ling-min21.Department of Life Science , Technology,Xinxiang Medical College,Xinxiang 453003,2.Laboratory of Morphology |
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| Abstract: | cDNA fragment of fertility gene MS2 from cotton was cloned by RT-PCR approach, it was highly homologous with relevant genes of Brassica napus and Arabidopsis thaliana. According to the principles of constructing RNAi vector, sense and antisense fragments of MS2 gene carrying restriction endonuclease recognition sites were amplified via PCR technique, ligated with the first intron of upland cotton chinase gene, then inserted into artificially modified plant expression vector pBI121, yielding RNAi vector pBGP... |
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| Keywords: | Cotton MS2 RT-PCR RNAi |
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