| 烤烟K326人工种子悬浮培养下体细胞胚胎的发生研究 |
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| 引用本文： | 王连华,卢江平,晋艳,白永富,张恒,马文广. 烤烟K326人工种子悬浮培养下体细胞胚胎的发生研究[J]. 种子, 2002, 0(4): 10-12 |
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| 作者姓名： | 王连华 卢江平 晋艳 白永富 张恒 马文广 |
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| 作者单位： | 云南省楚雄州烟科所,楚雄,675000;中国烟草育种研究,南方,中心,玉溪,653100 |
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| 基金项目： | 云南省烟草公司科技计划资助项目“烟草人工种子研制与应用”内容之一 |
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| 摘 要： | 通过对烤烟K326不同外植株、不同培养基及影响烟草体细胞胚发生因素的研究。结果表明：烤烟K326产生愈伤组织的百分率因不同外植株而异，在含2，4－D 1.0mg/L的MS固体培养基上产生愈伤组织的百分率分别为52％、26％、4％，而在含NAA0.1mg/L和KT2.0mg/L的MS去分化培养基上产生愈伤组织的百分率分别为63％、44％、5％；体细胞胚发育是不同步的；一定浓度的2，4－D对烤烟体胚发生是必须的，但高浓度2，4－D将抑制体胚的形成；2％蔗糖比3％蔗糖利于烤烟K326体胚发生。
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| 关 键 词： | 人工种子 悬浮培养 烤烟K326 体细胞胚 愈伤组织 |
Study on the Development Flue-cured Tobacco K326 Artificial Seed of Somatic Embryos in Floating Culture |
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| Wang Lianhua Lu Jiangping Jin Yan Bai Youfu Zhang Heng Ma Wenguang. Study on the Development Flue-cured Tobacco K326 Artificial Seed of Somatic Embryos in Floating Culture[J]. Seed, 2002, 0(4): 10-12 |
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| Authors: | Wang Lianhua Lu Jiangping Jin Yan Bai Youfu Zhang Heng Ma Wenguang |
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| Abstract: | Study on the influence of media, factors and explant on the percentage of callus formation for flue cured tobacco K326. It showed that the percentage of callus formation for flue cured tobacco K326 was different, in variant explants. The percentage of callus formation was respective 52%,26% and 4% in MS media containing, 2,4 D 1.0mg/L, it was respective 63%,44% and 5% in MS polarication media containing NAA 0.1mg/L and KT 2.0mg/L and KT 2.0mg/L. The development of somatic embryos was in different stages; The suitable degree 2,4 D was essential to developing flue cured tobacco of somatic embryos, while high degree 2,4 D would restrain flue cured tobacco of somatic embryos.2% saccharose was much better than 3% on development flue cured tobacco K326 of somatic embryos. |
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| Keywords: | Flue cured tobacco K326 Callus formation Somatic embryos |
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