| 不同固定剂及固定时间对三角帆蚌DNA提取效果比较 |
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| 引用本文： | 许巧情,董明钢. 不同固定剂及固定时间对三角帆蚌DNA提取效果比较[J]. 淡水渔业, 2009, 39(5) |
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| 作者姓名： | 许巧情 董明钢 |
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| 作者单位： | 长江大学动物科学学院,湖北荆州,434025;长江大学动物科学学院,湖北荆州,434025 |
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| 基金项目： | 湖北省教育厅科研项目，湖北省自然科学基金 |
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| 摘 要： | 以三角帆蚌(Hyriopsis cumingii)为对象,研究了无水乙醇,95%乙醇,4%多聚甲醛,福尔马林溶液,TE缓冲液5种固定剂不同固定时间对三角帆蚌斧足DNA提取质量及PCR扩增的影响。结果显示95%乙醇固定后提取的三角帆蚌基因组DNA降解少,DNA浓度高,OD260/OD2 8 0在1.8~2.0之间,OD260/OD230在1.5左右,表明95%乙醇固定后三角帆蚌基因组DNA的提取质量最好,再依次为无水乙醇、福尔马林溶液、4%多聚甲醛,DNA提取质量最差的是用TE固定的三角帆蚌。
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| 关 键 词： | 三角帆蚌(Hyriopsis cumingii) 基因组DNA 固定剂 PCR扩增 |
Comparative study of extraction genomic DNA with different preservations in Hyriopsis cumingii |
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| XU Qiao-qing,DONG Ming-gang. Comparative study of extraction genomic DNA with different preservations in Hyriopsis cumingii[J]. Freshwater Fisheries, 2009, 39(5) |
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| Authors: | XU Qiao-qing DONG Ming-gang |
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| Affiliation: | XU Qiao-qing,DONG Ming-gang(College of Animal Science,Yangtze University,Jingzhou,Hubei 434025) |
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| Abstract: | Freshwater pearl mussel,Hyriopsis cumingii,commonly called triangle mussel,belonging to family Unionindae,is mainly distributed in large lakes and rivers,and is rich in the five largest freshwater lakes in China:Poyanghu Lake,Dongtinghu Lake,Taihu Lake,Chaohu Lake and Hongzehu Lake.Extraction of genomic DNA from preserved specimen is a key step to study phylogeny and molecular genetics.This paper compared the effects of different preservatives on extraction of genomic DNA in a pearl-rearing shell,H.cumingii... |
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| Keywords: | Hyriopsis cumingii genomic DNA extraction preservation PCR ampilification |
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