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pH-sensitivity of YFP provides an intracellular indicator of programmed cell death
Authors:Bennett Young  Raymond Wightman  Robert Blanvillain  Sydney B Purcel  Patrick Gallois
Institution:1.Faculty of Life Sciences, Michael Smith Building,University of Manchester,Manchester,UK;2.Biochimie et Physiologie Moléculaire des Plantes, CNRS UMR 5004,Montpellier Cedex 1,France
Abstract:

Background  

Programmed cell death (PCD) is an essential process for the life cycle of all multicellular organisms. In higher plants however, relatively little is known about the cascade of genes and signalling molecules responsible for the initiation and execution of PCD. To aid with the discovery and analysis of plant PCD regulators, we have designed a novel cell death assay based on low cytosolic pH as a marker of PCD.
Keywords:
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