注射L-精氨酸和环磷酰胺对杂色鲍血清NO水平、NOS活性及免疫指标的影响

对杂色鲍(Haliotis diversicolor)分别按每kg体质量注射250mg、500mg和1000mg的L-精氨酸和5mg、10mg、20mg的环磷酰胺。每隔5d足部肌肉注射1次,共注射3次。第16天检测其血清中NO含量以及NOS活性的变化情况。同时采用注射环磷酰胺的方法(剂量为10mg/kg体质量),对杂色鲍血清中NOS活性进行负调控,分别在注射后3h、6h、12h、24h、48h、96h对血清NO水平和血清NOS、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)以及溶菌酶(LZM)活性进行测定,并探讨它们之间的相关性。结果显示,注射500mg/kg的L-精氨酸可以显著提高实验鲍血清中NO水平和NOS活性,而注射10mg/kg的环磷酰胺则可以显著降低实验鲍血清中NO水平和NOS活性。血清中NOS活性与ACP、AKP以及LZM等活性的相关系数分别为0.8074、0.8292和0.7408,相关显著(P<0.05)或极显著(P<0.01),NO与SOD及LZM的相关系数分别为0.9302和0.9413,相关极显著(P<0.01)。实验结果为人工调控鲍体内的NO含量、增强其自身的非特异性免疫功能,从而提高鲍的抵抗疾病的能力提供了科学依据,同时也证明NO和NOS可以作为评价鲍免疫功能强弱的指标。

Effects of injecting L-Arginine and cyclophosphamide on serum NO concentration, NOS activity and other immune index in Haliotis diversicolor

With the development of abalone culture,more and more epidemic disease broke out frequently,which made heavy losses.The common drugs and methods used in controlling epidemic disease had no obvious effect.The ability of aquatic animal to resist the disease can be enhanced by using immune technique to stimulate their immune system,and it proved to be a fundamental way to control the disease.The nitric oxide(NO),which is produced during the conversion of L-arginine to L-citrulline by NO synthase(NOS),is a free radical biological messenger with numerous functions, including regulation of vascular tone,cellular signaling,being ubiquitous in all living organs and tissues.It takes part in many important physiological and pathological reactions,and can kill the bacteria, fungi,parasite or virus non-specif ically with improving the non-specif ic immune function.In recent years,studies on NO have been gotten on very quickly in life science f ield,but little research has been focused on NO of the abalone.To study the effects of injection L-Arginine and Cyclophosphamide on serum NO concentration and NOS activity of Haliotis diversicolor Reeve,different dose of L-Arginine(0,250,500,1 000 mg per kg body weight abalone)and Cyclophosphamide(0,5,10,20 mg per kg body weight abalone)were respectively injected into the foot muscle of Haliotis diversicolor every 5 days for three times in present study.The NO concentration and NOS activity in the serum were detected on the 16th day.After injection of Cyclophosphamide(with the dose of 10 mg/kg at 3 h,6 h,12 h,24 h,48 h and 96 h after injectien),the NO,NOS,SOD,ACP,AKP and LZM activities were examined,and the correlation between them was analyzed.The results showed that compared to control group, L-Arginine(with dose 500 mg/kg)could induce an up-regulation the NO concentration and NOS activity,but Cyclophosphamide(with dose 10 mg/kg)could decrease the NO concentration and NOS activity.And the correlation coeff icient between NOS and AKP,ACP,LZM activities was 0.8074,0.8292 and 0.7408 respectively, which had significant correlation or marked significant correlation.The correlation coefficient between NO and SOD, LZM activities was 0.9302 and 0.9413 respectively.In practice,in order to enhance the non-immunology function of abalone and strengthen its ability to resist the disease,the NO in vivo could be induced and regulated artif icially by using the L-Arginine or Cyclophosphamide.And the NO/NOS could be regarded as the immune index to evaluate the immune function of abalone.