蛴螬感染嗜菌异小杆线虫后酶活性和能源物质含量变化

为探究昆虫病原线虫对蛴螬的致病机理，测定了蛴螬感染嗜菌异小杆线虫沧州品系 Heterorhabditis bacteriophora Cangzhou strain后其酚氧化酶（PO）、羧酸酯酶（CarE）活性以及血淋巴蛋白、海藻糖含量的变化。结果表明，线虫侵染后蛴螬 PO 和 CarE 活性大致表现出“升高-峰值-下降”的变化过程，华北大黑鳃金龟 Holotrichia oblita Faldermann、暗黑鳃金龟 H. parallela Motschulsky 和铜绿丽金龟 Anomala corpulenta Motschulsky 3种金龟子2龄幼虫PO活性分别在24、12、36 h达到最大并显著高于对照，CarE活性则分别在48、36、48 h达到最大且显著高于对照。注射线虫悬浮液至铜绿丽金龟3龄幼虫血腔后20 h，血淋巴蛋白含量最高且显著高于对照，28 h含量最低且显著低于对照；海藻糖含量在注射后20 h达到最高但与对照差异不显著，28 h含量最低且显著低于对照。两种酶活性以及能源物质含量的变化，表明嗜菌异小杆线虫入侵对蛴螬免疫系统和代谢过程产生了一定影响，也为揭示昆虫病原线虫对蛴螬的致病机理提供了一定的试验数据。

Effects of Heterorhabditis bacteriophora Infection on Enzymes Activities and Energy Substances Content in White Grubs

To explore the pathogenesis of infection with Heterorhabditis bacteriophora in white grubs, the activity of phenoloxidase （PO） and carboxylesterase （CarE） in hemolymph, as well as protein and trehalose content, between infected grubs and control were observed. The change tendencies of the PO and CarE activities in infected grubs were similar. At 24, 12, 36 h after infection, the PO activities in the 2nd instar larvae of Holotrichia oblita Faldermann, H parallela Motschulsky and Anomala corpulenta Motschulsky reached maximum and were significantly higher than each control （P＆lt;0.05）, respectively. Similarly, after 48, 36, 48 h of infection, CarE activities in the three white grubs ran to maximum respectively, significantly higher than control （P＆lt;0.05）. The protein content in hemolymph of 3rd instar A. corpulenta larvae increased greatly after 20 h of infection then decreased dramatically at 28 h. The trehalose content reached the highest at 20 h, and lowest at 28 h. These changes were significant difference against the control （P＆lt;0.05）. Taken together, it indicated that H. bacteriophora could affect the immune system and metabolic processes in white grubs. These results laid a foundation for further＆amp;nbsp;analysis of pathogenesis of infection with H. bacteriophora in white grubs.