| 甘蔗愈伤组织分化绿苗内源激素变化规律研究 |
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| 引用本文： | 肖关丽,杨清辉,李富生,杨生超.甘蔗愈伤组织分化绿苗内源激素变化规律研究[J].西南农业大学学报,2002,24(4):337-339. |
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| 作者姓名： | 肖关丽 杨清辉 李富生 杨生超 |
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| 作者单位： | 云南农业大学农学与生理技术学院 云南昆明650201
(肖关丽，杨清辉，李富生)，云南农业大学农学与生理技术学院 云南昆明650201(杨生超) |
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| 基金项目： | 云南省“九五”攻关资助项目 |
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| 摘 要： | 对甘蔗愈伤组织分化过程中的内源激素进行了测定，分析，结果表明：甘蔗愈伤组织分化过程中，伴随愈伤口分化过程中的变化，内源激素发生一系列的变化，IAA的强烈合成与胚性细胞的发生相联系，当胚性细胞大量发生时，IAA的合成达到峰值，当CTK达到峰值时，分化苗大量发生，而GA1 3的合成和分化苗的长高密切相关，GA1 3在分化20d才达到峰值，分化20d后,各种内源激素含量下降并趋向稳定。
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| 关 键 词： | 甘蔗 愈伤组织 内源激素 变化规律 幼苗分化 组织培养 |
| 文章编号： | 1000-2642(2002)04-0337-03 |
| 修稿时间： | 2001年12月4日 |
DYNAMICS OF ENDOGENOUS HORMONES IN THE COURSE OF SHOOT DIFFERENTIATION FROM CALLI OF SUGARCANE |
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| XIAO Guan-li,YANG Qing-hui,LI Fu-sheng,YANG Sheng-chao.DYNAMICS OF ENDOGENOUS HORMONES IN THE COURSE OF SHOOT DIFFERENTIATION FROM CALLI OF SUGARCANE[J].Journal of Southwest Agricultural University,2002,24(4):337-339. |
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| Authors: | XIAO Guan-li YANG Qing-hui LI Fu-sheng YANG Sheng-chao |
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| Abstract: | The levels of endogenous hormones in sugarcane were monitored and analyzed in the course of callus differentiation and shoot regeneration. The results showed that active synthesis of IAA was associated with the induction of embryonic cells and IAA synthesis reached its maximum when a great number of embryonic cells were induced. The occurrence of the maximum of CTK coincided with that of differentiated shoots. The synthesis of GA 1+3 was found to be closely related to the growth in height of the regenerated shoots and reached its maximum 20 days after shoot differentiation. Thereafter, the contents of various endogenous hormones declined and gradually became stable. |
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| Keywords: | sugarcane shoot differentiation endogenous hormone tissue culture |
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