淫羊藿属6种植物的花粉活力与柱头可授性

用TTC法测定淫羊藿属6种9份材料花粉活力,用联苯胺-过氧化氢法测定其柱头可授性,探讨淫羊藿属植物的花粉活力及柱头可授性变化规律,为阐明其有性繁殖特性和杂交育种积累资料。结果表明:不同种类和同种不同居群之间,花粉活力差异较大,最高的为天全淫羊藿,可达87.8%,最低的为望鱼居群的粗毛淫羊藿,其峰值为48.5%;所有材料的花粉活力变化趋势相似,均在开花后1~2 d内达到其峰值,然后逐渐下降,花粉寿命为3~5 d;所有材料均在开花后1~2 d内柱头可授性最强,可授期为3~5 d,其可授性强弱和变化规律比较一致,说明淫羊藿属植物的柱头特征是比较保守的;6种淫羊藿属植物人工授粉的最佳时期是开花后1~2 d。

Pollen Viability and Stigma Receptivity of Six Epimedium Species

To provide the basal data for artificial cross breeding of six Epimedium species,pollen viabilities of six Epimedium species were evaluated using TTC(2,3,5-triphenyl tetrazolium chloride),while stigma receptivities were measured by a benzidine-H2O2 method.Results indicated that Pollen viabilities were different among species or populations.The highest pollen viability was that of E.flavum(87.8%),while the least was that of E.acuminatum(48.5%) from Wangyu.Pollen viabilities of all materials were relatively high in 1 to 2 days after blooming,then decreased gradually.The longevity of pollen was about 3 to 5 days.The stigma of all materials had receptivity within 3 days or 5 days after blooming,but it was stronger in 1 to 2 days than in other days.Stigma receptivity and its change tendency were similar,indicating that the stigma feature of Epimedium species was conservative.The optimum artificial pollination time of these six species was 1 to 2 days after blooming.