香椿嫩叶中黄酮类化合物的提取

为确定香椿嫩叶中黄酮类化合物的提取工艺,用乙醇溶液浸提,结合超声波辅助方法,萃取香椿嫩叶中的黄酮类化合物,探讨了影响提取率的主要因素,最后用正交法确定了较好的提取工艺,并用方差分析进行了显著性检验。结果表明：在１．００ｇ样品中,加５０ｍＬ体积分数为５０％的乙醇水溶液提取剂,浸泡２４ｈ后,超声波辅助萃取４５ｍｉｎ,连续萃取２次,总浸出率可达９６．４７％。香椿嫩叶中的叶片黄酮提取率为７．４５％,叶柄为

Extractingflavonoid compounds from Toona sinensis leaves

Using a method applying alcohol soltion as extraction solvent in cooperation with ultrasonic wave in extraction techniques of flavonoid compounds in Toona sinensis leaves,discussing the primary factors which influences extraction ratio,and analyzing preferable extraction techniques by variance analysis and orthogonal design,the results show that a general extraction ratio reaches 96 47% after soaking 1 00 g sample in 50 mL 50% alcoholsolution for 24 h with application of ultrasonic wave for 45 min and repeating again.The extracting ratio of flavonoids is 7 45% in the leaves and 5 40% in the leafstalk.The volume percentage of alcohol is a predominant factor for extraction ratio.Application time of ultrasonic wave have certain influence while solvent quantity shows little influence on extraction ratio.