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金铁锁总皂苷提取工艺研究
引用本文:吕小梨 王华磊 赵致 周英 罗春丽 李金玲 刘红昌 徐,绯.金铁锁总皂苷提取工艺研究[J].中国农学通报,2011,27(5):470-474.
作者姓名:吕小梨 王华磊 赵致 周英 罗春丽 李金玲 刘红昌 徐  
作者单位:贵州大学中药材研究所,贵阳,550025;贵州省药用植物繁育与种植重点实验室,贵阳,550025
基金项目:贵州省科学技术基金项目
摘    要:采用正交设计,以齐墩果酸为对照品,采用5%香草醛冰醋酸-高氯酸显色剂,以总皂苷提取率为指标来确定金铁锁的最佳提取工艺。结果表明,最佳提取工艺如下:提取温度为70℃、乙醇浓度为80%、提取时间90 min、料液比1:8,该方法成本低、结果稳定、重复性好。

关 键 词:临界值    临界值
收稿时间:2010/12/6 0:00:00
修稿时间:1/2/2011 12:00:00 AM

Extraction Process of the Saponins in psammosilene tunicoides W.C. WU et C.Y.Wu.
Lv Xiaoli,Wang Hualei,Zhao Zhi,Zhou Ying,Luo Chunli,Li Jinling,Liu Hongchang,Xu Fei.Extraction Process of the Saponins in psammosilene tunicoides W.C. WU et C.Y.Wu.[J].Chinese Agricultural Science Bulletin,2011,27(5):470-474.
Authors:Lv Xiaoli  Wang Hualei  Zhao Zhi  Zhou Ying  Luo Chunli  Li Jinling  Liu Hongchang  Xu Fei
Institution:Lv Xiaoli 1,2,Wang Hualei 1,Zhao Zhi 1,Zhou Ying 1,Luo Chunli 1,Li Jinling 1,Liu Hongchang 1,Xu Fei 1,2 ( 1 Research Institute for Chinese Medicinal Material of Guizhou University,Guiyang 550025,2 Guizhou Key Laboratory of Propagation and Cultivation on Medicinal Plants,Guiyang 550025)
Abstract:The orthogonal design was used to study the optimum process of extracting total saponins in Psammosilene tunicoides W.C. WU et C. Y. Wu. The oleanolic acid was selected as comparison product and 5% vanillin-acetic acid-HClO solution was selected as color-producing reagent. As an indicator, the extraction rate of total saponins was used to indicate the best extraction process. The optimum technological conditions were as follows: extraction temperature 70℃, ethanol concentration 80%, treat time 90 min, ratio of solid to liquor at 1:8. The optimized process had the advantages of low cost, stable and the excellent repeatability.
Keywords:Psammosilene tunicoides W  C  WU et C  Y  Wu  total saponins  extraction technology  
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