| 磷脂酶D对拟南芥抗冻性的影响 |
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| 引用本文： | 赵鹏,王道龙.磷脂酶D对拟南芥抗冻性的影响[J].中国农学通报,2011,27(6):107-111. |
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| 作者姓名： | 赵鹏 王道龙 |
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| 作者单位： | 1. 中国农业科学院农业资源与农业区划研究所,北京,100081;中山火炬职业技术学院,广东中山,528436
2. 中国农业科学院农业资源与农业区划研究所,北京,100081 |
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| 摘 要： | 为了探索磷脂酶D（PLD）在调控植物抗冻性中的作用，进一步揭示植物抗冻机理和磷脂低温信号转导机制的研究。笔者应用人工气候霜箱，对PLDγ1、PLDγ3基因分别被敲除的拟南芥突变体及野生型材料，进行低温驯化和冻害胁迫处理。试验发现，这2个基因的敲除型无论是经过还是未经过低温驯化冻害处理的离子渗漏率都与相同处理野生型拟南芥的离子渗漏率无显著差异。试验结果表明，PLDγ1和PLDγ3这2个基因既未参与组成型调控植物的抗冻性，也未参与低温信号转导过程。
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| 关 键 词： | C4途径 C4途径 PEPC 抗旱生理 转基因育种 |
| 收稿时间： | 2010/8/30 0:00:00 |
| 修稿时间： | 2011/1/10 0:00:00 |
Study on the Effect of Phospholipase D on the Freezing Tolerance of Arabidopsis thaliana |
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| Zhao Peng,Wang Daolong.Study on the Effect of Phospholipase D on the Freezing Tolerance of Arabidopsis thaliana[J].Chinese Agricultural Science Bulletin,2011,27(6):107-111. |
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| Authors: | Zhao Peng Wang Daolong |
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| Institution: | Zhao Peng1,2,Wang Daolong1(1Institute of Agricultural Resources and Regional Planning,CAAS,Beijing 100081,2Zhongshan Torch Polytechnic,Zhongshan Guangdong 528436) |
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| Abstract: | The experiment was purposely to research the effect of phospholipase D on the freezing tolerance of Arabidopsis thaliana,reveal the mechanism of plants freezing tolerance and phospholipid signal transduction. Arabidopsis thaliana mutantPLDγ 1 orPLDγ 3 was deficient and wild-type was used as materials. The two mutants and wild-type were cold acclimated and freezing stressed using a climate chamber. There were no significant differences between the ion leakage of the mutants and wild-type whether cold acclimation freeze or non-acclimation freeze. The results showed thatPLDγ 1 andPLDγ 3 neither mediate regulation of constitutive freezing tolerance nor low temperature signaling. |
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| Keywords: | phospholipase D Arabidopsis thaliana cold acclimation signal transduction |
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