| 烟草CMS相关基因orf25的生物信息学分析 |
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| 引用本文： | 周,玮 黄,河 周伯楠 刘齐元.烟草CMS相关基因orf25的生物信息学分析[J].中国农学通报,2011,27(5):312-316. |
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| 作者姓名： | 周 玮 黄 河 周伯楠 刘齐元 |
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| 作者单位： | 1. 湖南农业大学生物安全科学技术学院,长沙,410128
2. 江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室,南昌,330045 |
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| 基金项目： | 国家自然科学基金项目，湖南农业大学大学生科技创新基金项目 |
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| 摘 要： | 线粒体基因orf25异常可能导致烟草细胞质雄性不育（CMS）。为探讨orf25基因导致CMS的可能机理，本文采用生物信息学方法，分析烟草雄性不育系‘MS革新3号’及其保持系中的orf25基因在其编码蛋白各结构层次上存在的差异。结果显示，不育系中orf25基因因碱基改变提前出现终止子从而导致其编码蛋白缺失结构域，这极可能成为干扰线粒体F(0)F(1)-ATP合成酶功能的关键因素，从而成为导致烟草‘革新3号’CMS的根本原因之一。
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| 关 键 词： | 干物质积累 干物质积累 |
| 收稿时间： | 2010/9/15 0:00:00 |
| 修稿时间： | 2010/10/26 0:00:00 |
The Bioinformatics Analysis of orf25 Gene Related to Tobacco Cytoplasmic Male Sterility |
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| Zhou Wei,Huang He,Zhou Bonan,Liu Qiyuan.The Bioinformatics Analysis of orf25 Gene Related to Tobacco Cytoplasmic Male Sterility[J].Chinese Agricultural Science Bulletin,2011,27(5):312-316. |
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| Authors: | Zhou Wei Huang He Zhou Bonan Liu Qiyuan |
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| Institution: | Zhou Wei 1,Huang He 1,Zhou Bonan 1,Liu Qiyuan 2 ( 1 College of Bio-safety Science and Technology,Hunan Agricultural University,Changsha 410128,2 College of Agronomy,Jiangxi Agricultural University/Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Nanchang 330045) |
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| Abstract: | It was reported that aberrant mitochondrial gene orf25 might cause tobacco cytoplasmic male sterility （CMS）. To understand the mechanism of CMS, the differences of orf25 gene between CMS line ‘ Gexin 3 ’ and its maintainer line were analyzed by bioinformatics at different structure levels of coding protein. The results showed that the terminal codon appeared early because of the base change in the orf25 gene, which led to the lack of the structural domain in coding protein. It might be the key factor that disturbed the function of F（0）F（1）-ATP synthetase in mitochondrion, and become one of the fundamental causes which led to CMS of tobacco. |
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| Keywords: | tobacco cytoplasmic male sterility (CMS) orf25 gene bioinformatics male sterile mechanism |
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