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| 荷斯坦牛Leptin基因外显子2多态性与繁殖力性状的相关性研究 | |
| 引用本文： | 李鹏,何举,赵国丽,宫艳斌,韩元,王丽,史远刚.荷斯坦牛Leptin基因外显子2多态性与繁殖力性状的相关性研究[J].中国奶牛,2011(6):4-7. |
| 作者姓名： | 李鹏 何举 赵国丽 宫艳斌 韩元 王丽 史远刚 |
| 作者单位： | 1. 宁夏正鑫牧业科技发展有限公司,银川750002;宁夏大学农学院,银川750021 2. 宁夏正鑫牧业科技发展有限公司,银川,750002 3. 宁夏大学农学院,银川,750021 4. 宁夏四正生物工程技术研究中心,银川,750200 5. 宁夏正鑫牧业科技发展有限公司,银川750002;宁夏四正生物工程技术研究中心,银川750200;宁夏大学农学院,银川750021 |
| 基金项目： | 现代农业(奶牛)产业技术体系建设项目 |
| 摘 要： | 以94头荷斯坦牛为试验材料,应用PCR-SSCP技术研究Leptin基因外显子2多态性与繁殖力的相关性。在Leptin基因外显子2的E2FB区上,经分析存在影响奶牛繁殖的多态位点,测序首次发现102nt处T突变为C。在平均产犊间隔上,AA与AT差异极显著（P〈0.01）;AT与TT差异极显著（P〈0.01）;A为优势等位基因,可作为荷斯坦牛育种中繁殖力的分子辅助标记。 |
| 关 键 词： | Leptin基因外显子2 胎间隔 繁殖力 荷斯坦牛 |
Study on Polymorphism in the Exon 2 of Leptin Gene and Its Relationship with Fertility Traits of Holstein Cows |
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| LI Peng,HE Ju,ZHAO Guo-li,GONG Yan-bin,HAN Yuan,WANG Li,SHI Yuan-gang.Study on Polymorphism in the Exon 2 of Leptin Gene and Its Relationship with Fertility Traits of Holstein Cows[J].China Dairy Cattle,2011(6):4-7. | |
| Authors: | LI Peng HE Ju ZHAO Guo-li GONG Yan-bin HAN Yuan WANG Li SHI Yuan-gang |
| Institution: | 1.Ningxia Sinogen Farming Sic-Tec Development Co.,Ltd.;2.Ningxia Syngen Bio-Engineering Research Center,Ningxia 750200; 3.Agriculture College of Ningxia University,Ningxia 750021) |
| Abstract: | PCR-SSCP technology were employed to analyze the correlation between Leptin gene＇s polymorphism and fertility traits in 94 Holstein cows.The polymorphism（T/C）in the Locus 102bp affecting the cows＇ fertility was firstly detected in the exon 2 area （E2FB）of Leptin gene,after Sequencing.For the average parity interval,there were significant differences between genotype AA and genotype AT（P〈0.01）,between genotype AT and genotype TT（P〈0.01）.Genotype A was dominant allele,which could be applied for the molecular genetic markers in fertility of Holstein cows. |
| Keywords: | Leptin gene＇s exon 2 Parity Interval Fertility Holstein |
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