家蚕核型多角体病毒几丁质酶基因

序列分析表明 :家蚕核型多角体病毒苏州株 (BmNPVsu)编码的ChiA基因的ORF为 16 5 6个核苷酸 ,编码 5 5 1个氨基酸。同源性分析表明 :杆状病毒编码的ChiA基因在DNA水平上、氨基酸水平上都具有较好的同源性 ,与灵菌的ChiA在氨基酸水平上的同源性达 47 8%～ 5 8 5 % ,推测杆状病毒编码的ChiA基因由细菌通过基因的水平转移而来。在杆状病毒ChiA的系统树中 ,舞毒蛾核型多角体病毒 (LdNPV)为一单独分枝 ,处于进化树的最根部 ,其余可分 2组 ,其中棉铃虫核型多角体病毒 (HaNPV)ChiA、谷实夜蛾核型多角体病毒 (HzNPV)ChiA为一组 ,其它杆状病毒的ChiA构成了第二组

Bombyx mori Nuclear Polyhedrosis Virus Chitinase Gene

The chitinase gene of Bombyx mori nuclear polyhedrosis virus Suzhou strain (BmNPVsu) was sequenced.The ORF of BmNPVsu Chi A was 1 656nt which encoded protein of 551 amino acid.Comparing with other chitinase,the homology of nucleotide and amino acid are much high.When the Chi A from baculoviruses were compared with the Chi A from Serratia marcescens ,the identity on amino acid level was 47.8%～58.5%,by which it is deduced that baculovirus acquired the chitinase gene from a bacterium via horizontal gene transfer.Independing branch of Lymantria dispar nuclear polyhedrosis virus (LdNPV) is on the root of phylogenetic tree,the others are divided into two groups,one group makes up of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) and Helicoverpa zea nuclear polyhedrosis virus (HzNPV),the second group is composed of the remains.