| H3N8亚型马流感病毒单克隆抗体的制备及鉴定 |
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| 引用本文： | 肖成蕊,宋战昀,杨立英,王伟利,孟庆峰,孟日增.H3N8亚型马流感病毒单克隆抗体的制备及鉴定[J].动物医学进展,2011,32(6):1-4. |
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| 作者姓名： | 肖成蕊 宋战昀 杨立英 王伟利 孟庆峰 孟日增 |
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| 作者单位： | 吉林出入境检验检疫局;吉林省磐石市动物检疫站; |
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| 基金项目： | 国家质检总局基金资助项目(2007IK026) |
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| 摘 要： | 以灭活马流感病毒(EIV)A/Equine/Jilin/1/1989(H3N8)为免疫原,免疫Balb/c小鼠,经常规细胞融合后,用血凝抑制试验(H1)和间接ELISA方法筛选获得3株(3C2、5G10和5A10)能稳定分泌H3N8亚型马流感病毒单克隆抗体(mAb)的杂交瘤细胞株.其中3C2和5G10为IgG2α,5A...
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| 关 键 词： | H3N8亚型马流感病毒 单克隆抗体 血凝素 特性鉴定 |
Preparation and Characterization of the Monoclonal Antibodies Against H3N8 Subtype Equine Influenza Virus |
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| XIAO Cheng-rui,SONG Zhan-yun,YANG Li-ying,WANG Wei-li,MENG Qing-feng,MENG Ri-zeng.Preparation and Characterization of the Monoclonal Antibodies Against H3N8 Subtype Equine Influenza Virus[J].Progress In Veterinary Medicine,2011,32(6):1-4. |
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| Authors: | XIAO Cheng-rui SONG Zhan-yun YANG Li-ying WANG Wei-li MENG Qing-feng MENG Ri-zeng |
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| Institution: | XIAO Cheng-rui1,SONG Zhan-yun1,YANG Li-ying2,WANG Wei-li1,MENG Qing-feng1,MENG Ri-zeng1(1.Jilin Entry-Exit Inspection and Quarantine Bureau,Changchun,Jilin,130062,China,2.Panshi Animal Quarantine Station of Jilin Province,Panshi,132300,China) |
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| Abstract: | Three monoclonal antibodies to equine influenza virus were obtained by fusing SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with A/Equine/Jilin/1/1989(H3N8),which were designated as 3C2,5G10 and 5A10 respectively.In which 3C2 and 5G10 as the IgG subclasses,5A10 for the IgM subclass antibody titer detection of ascites,3C2,and 5G10 to 1∶64 000,5A10 is 1∶32 000.Both HI and Western blot results showed that three monoclonal antibodies are EIV hemagglutinin-specific monoclonal antibodies.By hemagglutination inhibition test(HI),indirect ELISA and Western blot assay,these monoclonal antibodies reacted specifically with EIV but not with H7N7 subtype EIV,equine arteritis virus(EAV),equine infectious anemia virus(EIAV),horse encephalitis virus(JEV).The results suggested that these monoclonal antibodies are very useful in diagnosing equine influenza. |
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| Keywords: | H3N8 subtype equine influenza virus monoclonal antibody hemagglutinin biological characteristic |
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