新疆主栽杏品种自交不亲和SFB基因的检测

 为系统鉴定新疆主栽杏品种自交不亲和SFB基因型，以新疆25个主栽杏品种为试材，利用蔷薇科保守序列设计引物对叶片基因组DNA进行SFB基因特异PCR扩增，筛选出有效的扩增引物；对成功扩增的杏品种的特异条带克隆测序，在GenBank上进行BLASTN比对，确定各品种的SFB基因型。结果显示：引物组合Ⅱ-1、Ⅳ-2，1-Ⅰ、1-Ⅱ对新疆杏品种的扩增效果最好，成功地在18个品种上扩增出了5种大小不同的条带，14种不同的基因型，其中两

Identification of Self-incompatibility SFB Genes for Apricot Cultivars in Xinjiang Area

In order to identify SFB gene associated with self-incompatibility of apricot cultivars in Xinjiang area systematically，five pairs of primers designed according to the conserved sequences of Rosaceae SFB were used for specific PCR amplification of 25 mainly apricot cultivars in Xinjiang. The specific fragments amplified successfully were cloned and sequenced. The results showed：The primer combinationⅡ-1、Ⅳ-2，1-Ⅰ、1-Ⅱ was the best optional；Five different size fragments and 14 different genes were cloned from 18 apricot cultivars. Searches for homology with our sequences publicly available DNA databases revealed 2 SFB gene fragments（SFB-36，SFB-42）had been deposited in the GenBank （Accession No：EF062342）as SFB-13，and the other 12 SFB gene fragments were novel SFB genes，temporarily named as：SFB-30，SFB-31，SFB-32，SFB-33，SFB-34，SFB-35，SFB-37，SFB-38，SFB-39，SFB-40，SFB-41 and SFB-43. Comparison of amino acid sequences of this 14 SFB gene fragments in apricot revealed three conserved regions and two high variability regions. Sixty-two SFB genes in Rosaceae