| 拟南芥叶绿体SSR引物在甘蓝上的应用 |
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| 引用本文： | 张扬勇,方智远,王庆彪,刘玉梅,杨丽梅,庄木,孙培田. 拟南芥叶绿体SSR引物在甘蓝上的应用[J]. 园艺学报, 2011, 38(3): 549-555 |
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| 作者姓名： | 张扬勇 方智远 王庆彪 刘玉梅 杨丽梅 庄木 孙培田 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所,北京,100081 |
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| 基金项目： | 国家自然科学基金，中央级公益性科研院所基本科研业务费专项，农业部园艺作物遗传改良重点开放实验室项目，农业部大宗蔬菜产业技术体系项目 |
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| 摘 要： | 根据拟南芥的叶绿体全基因组序列，搜索获得89个叶绿体基因组SSRs（cpSSRs），平均每1.736 kb出现1个SSR。针对这些SSR设计cpSSR引物58对，其中32对能在甘蓝上应用。5对引物在甘蓝C300、C322和波里马油菜N478、N479间有多态性，这些片段均位于基因内含子区或基因间隔区。测序结果显示单核苷酸A/T重复数从8个到13个不等。同源性比较表明C300、N479之间的同源性大于两者与拟南芥之间的同源性。
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| 关 键 词： | 甘蓝 叶绿体SSR 波里马油菜 多态性 |
Utility of Arabidopsis Chloroplast Simple Sequence Repeat (cpSSR) Primers in Cabbage(Brassica oleracea L.var.capitata L.) |
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| ZHANG Yang-yong,FANG Zhi-yuan,WANG Qing-biao,LIU Yu-mei,YANG Li-mei,ZHUANG Mu,SUN Pei-tian. Utility of Arabidopsis Chloroplast Simple Sequence Repeat (cpSSR) Primers in Cabbage(Brassica oleracea L.var.capitata L.)[J]. Acta Horticulturae Sinica, 2011, 38(3): 549-555 |
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| Authors: | ZHANG Yang-yong FANG Zhi-yuan WANG Qing-biao LIU Yu-mei YANG Li-mei ZHUANG Mu SUN Pei-tian |
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| Affiliation: | ZHANG Yang-yong*,FANG Zhi-yuan,WANG Qing-biao,LIU Yu-mei,YANG Li-mei,ZHUANG Mu,and SUN Pei-tian (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
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| Abstract: | According to the Arabidopsis thaliana chloroplast whole genome sequence,89 chloroplast SSRs(cpSSRs)were searched,with an average of 1.736 kb per SSR. Thirty-two pairs of primers,out of 58 designed primers got effective amplification in cabbage. Between cabbage C300,C322 and rapeseed polima N478,N479,five primers showed polymorphism,which located in intron or intergenic region. The number of single nucleotide repeat A/T ranged from 8 to 13. Homology between C300 and N479 was greater than the homology between... |
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| Keywords: | Cabbage cpSSR polima rapeseed polymorphism |
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