甘薯和Ipomoea lacunosa的种间体细胞杂种植株再生及鉴定

用ＰＥＧ融合法融合甘薯品种高系１４号和近缘野生种Ｉｐｏｍｏｅａ ｌａｃｕｎｏｓａ的原生质体，将融合原生质体培养在含有０．０５ｍｇ／Ｌ２，４－Ｄ和０．５ｍｇ／Ｌ ＫＴ的ＭＳ培养基上，愈伤组织迅速增殖。将其中的７０个愈伤组织培养在添加３．０ｍｇ／Ｌ ＢＡＰ的ＭＳ培养基上，并进一步培养在ＭＳ基本培养基上，获得９株再生植株。过氧化物酶同工酶、酯酶同工酶和ＲＡＰＤ分析表明，其中２株再生植株（ＫＬ１和ＫＬ３）

Regeneration and Identification of Interspecific Somatic Hybrid Plants between Sweet Potato and Ipomoea lacunosa

Protoplasts of sweet potato cv. Kokei No. 14 and Ipomoea lacunosa were fused by PEG method. Fused protoplasts were cultured in a modified MS medium containing 0. 05 mg/ L 2, 4-D and 0. 5 mg/L kinetin. First cell division occurred within 4~5 days. And 12 weeks af ter plating, the obtained small calli up to 1~2 mm in diameter were transferred to MS medium supplemented with 0. 05 mg/L 2, 4-D and 0. 5 mg/L kinetin, resulting in rapid callus prolifera tion. After 70 calli of them were cultured on MS medium supplemented with 3. 0 mg/L BAP and further on the basal medium, 9 regenerated plants were obtained. The results from the analysis of peroxidase isozymes, esterase isozymes, and random amplified polymorphic DNA(RAPD) in dicated that 2 of 9 regenerated plants (KL1 and KL3) were interspecific somatic hybrids of Kokei No. 14 and I. lacunoxa.