| 广西巴马小型猪SLA-DQB基因外显子2的多态性研究 |
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| 引用本文： | 陈江伟,郭亚芬,兰干球,范晶,谢丽丽,王爱德,李柏.广西巴马小型猪SLA-DQB基因外显子2的多态性研究[J].广西农业科学,2010,41(8):824-826. |
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| 作者姓名： | 陈江伟 郭亚芬 兰干球 范晶 谢丽丽 王爱德 李柏 |
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| 作者单位： | 广西大学动物科学技术学院,南宁,530005 |
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| 基金项目： | 广西科技基础条件平台建设项目 |
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| 摘 要： | 为探讨广西巴马小型猪SLA-DQB基因外显子2的多态性,以其耳组织为试验材料,经PCR扩增获得广西巴马小型猪SLA-DQB外显子2片段,片段大小为273 bp,含有1个RsaI酶切位点和5个HaeIII酶切位点,基因型分别为27bp/246 bp和84 bp/83 bp/7 bp/16 bp/29 bp/54 bp。采用PCR-RFLP对30头广西巴马小型猪SLA-DQB基因外显子2的PCR扩增产物进行多态性分析,发现广西巴马小型猪封闭群内S,LA-DQB基因外显子2无多态性位点;而克隆测序及同源性比较分析表明,广西巴马小型猪SLA-DQB基因外显子2序列与普通猪（Sus Scrofa）、人类（Homo sapiens）的同源性分别为94.1%、79.1%。
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| 关 键 词： | 广西巴马小型猪 SLA-DQB基因 外显子2 PCR-RFLP |
Polymorphism of exon 2 in swine leukocyte antigen (SLA) DQB gene in Guangxi Bama minipig |
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| CHEN Jiang-wei,GUO Ya-fen,LAN Gan-qiu,FAN Jing,XIE Li-li,WANG Ai-de,LI Bai.Polymorphism of exon 2 in swine leukocyte antigen (SLA) DQB gene in Guangxi Bama minipig[J].Guangxi Agricultural Sciences,2010,41(8):824-826. |
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| Authors: | CHEN Jiang-wei GUO Ya-fen LAN Gan-qiu FAN Jing XIE Li-li WANG Ai-de LI Bai |
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| Institution: | (College of Animal Science and Technology,Guangxi University,Nanning 530005,China) |
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| Abstract: | The present experiment was undertaken to study the polymorphism of exon 2 in swine leukocyte antigen（SLA） DQB gene in Guangxi Bama minipig using PCR-RFLP technique.The ear of minipig was used as sample to isolate DNA.The amplified fragment of PCR-product SQL-DQB exon 2 was 273 bp,which contained one restriction site for Rsa Ⅰand five for HaeⅢ restriction enzymes with genotype of 27bp/246bp and 84bp/83bp/7bp/16bp/29bp/54bp.The analysis of amplified fragment showed that there is no polymorphism existing amongst exon 2 in SLA-DQB gene in Guangxi Bama minipig closed group.After sequencing and homology analysis,it was found that the exon 2 in SLA-DQB gene in Guangxi Bama minipig showed 94.1 and 79.1% homology with Sus Scrofa and Homo sapiens,respectively. |
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| Keywords: | PCR-RFLP |
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