肌肉生成抑制素和抑制素融合表达质粒的构建和表达

以乙肝表面抗原S基因为载体，分别将肌肉生成抑制素N端基因片段和抑制素N端基因片段分别插入S基因C端和第112～113 氨基酸残基密码子之间，构建MSTN-INH融合表达质粒pVAX-SMI。酶切和测序鉴定表明，重组质粒pVAX-SMI构建成功。脂质体法将pVAX-SMI转染HeLa细胞，Western Blot检测表达产物的免疫原性。结果表明，融合目的蛋白成功在HeLa细胞中表达，并具有免疫原性。

Construction and Expression of Myostatin-Inhibin DNA Vaccine pVAX-SMI

Two DNA fragments encoding N-term of MSTN and INH respectively were incorporated into coding region of hepatitis B virus S gene at C-term and middle(between amino acid position 112 and 113) to construct a myostatin expression vector pVAX-SMI. The plasmid pVAX-SMI was identified by restriction endonuclease digestion and sequencing. pVAX-SMI was transfected into HeLa cells and the recombinant protein was detected by Western Blot. The result showed that the plasmid could express recombinant MSTN and INH with immunogenicity successfully. The pVAX-SMI expression vector was constructed successfully.