| H5N1亚型禽流感病毒新疆株NA基因的克隆及序列分析 |
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| 引用本文： | 沙依兰古丽,李曦,崔尚金,成进,符子华,盛卓君,汪萍,巴格德力,黄大松. H5N1亚型禽流感病毒新疆株NA基因的克隆及序列分析[J]. 动物医学进展, 2007, 28(12): 30-35 |
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| 作者姓名： | 沙依兰古丽 李曦 崔尚金 成进 符子华 盛卓君 汪萍 巴格德力 黄大松 |
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| 作者单位： | 新疆畜牧科学院兽医研究所,新疆乌鲁木齐,830001;中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001;新疆自治区兽医防疫监督总站,新疆乌鲁木齐,830000 |
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| 基金项目： | 新疆自治区科技厅重点项目 |
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| 摘 要： | 利用RT-PCR技术对3株H5N1亚型高致病性禽流感病毒新疆株的NA基因进行了扩增,分别得到了3个毒株的NA基因,序列分析表明,3个毒株NA的全长核苷酸序列均为1 380 bp.同源性分析表明,3个分离株之间的核苷酸同源性在95.1%～97.9%之间;与来自青海、浙江、广东、东南亚等不同地区水禽禽流感病毒毒株的同源性为90.3%～98.8%;与分离自香港、浙江、越南等地区的流感病毒同源性为86.7%～97.9%.
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| 关 键 词： | 禽流感病毒 H5N1 NA基因 序列分析 |
| 文章编号： | 1007-5038（2007）12-0030-06 |
| 收稿时间： | 2007-09-21 |
| 修稿时间： | 2007-09-21 |
Cloning and Sequence Analysis of NA Gene of H5N1 Avian influenza virus Strains from Xinjiang |
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| SHAYILAN Guli,LI Xi,CUI Shang-jin,CHENG Jin,FU Zi-hua,SHENG Zhuo-jun,WANG Ping,BAGE Deli,HUANG Da-song. Cloning and Sequence Analysis of NA Gene of H5N1 Avian influenza virus Strains from Xinjiang[J]. Progress In Veterinary Medicine, 2007, 28(12): 30-35 |
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| Authors: | SHAYILAN Guli LI Xi CUI Shang-jin CHENG Jin FU Zi-hua SHENG Zhuo-jun WANG Ping BAGE Deli HUANG Da-song |
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| Abstract: | The NA gene of A/Gs/XJ/10(H5N1),A/Duck/XJ/4(H5N1)and A/CH/XJ/5(H5N1)were amplified by RT-PCR and the products were cloned into pMD18-T vector respectively.The nucleotide sequences of the NA gene were all 1380bp in length.The sequence analyses showed that the nucleotide homology of Xinjiang three strains was 95.1%-97.9%.Comparison with the strains from wild birds of Qinghai,Zhejiang,Guangdong,HongKong and southeastern Asia indicated that the nucleotide homology was 90.3%-98.8%.Comparison with the human strains from Hong Kong,Vietnam and Zhejiang revealed that the nucleotide homology was 86.7%-97.9%. |
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| Keywords: | Avian influenza virus H5N1 NA gene sequence analysis |
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