Fasciation in Crassula argentea: molecular identification of phytoplasmas and associated antioxidative capacity |
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| Authors: | Yaser Hassan Dewir Ayman Faisal Omar Yaser Mohamed Hafez Mohammed El-Sayed El-Mahrouk Rasha Yousef Mourad |
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| Affiliation: | 1.Plant Production Department, College of Food and Agriculture Sciences,King Saud University,Riyadh,Saudi Arabia;2.Department of Horticulture, Faculty of Agriculture,Kafrelsheikh University,Kafr El-Sheikh,Egypt;3.Department of Agricultural Botany, EPCRS Excellence Center, Plant Pathology and Biotechnology Laboratory, Faculty of Agriculture,Kafrelsheikh University,Kafr El-Sheikh,Egypt |
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| Abstract: | The present study reports on phytoplasma induced fasciation in Crassula argintea (Crassulaceae). DNA was extracted from symptomless and fasciated tissues and amplified by nested PCR using universal primers P1/P7 followed by R16F2n/R16R2 produced amplicons of 1.2 Kb. The nucleotide sequence analyses of the amplicons indicated that fasciated plants were infected by phytoplasma. Phylogenetic analysis placed the Crassula fasciation phytoplasmas in 16SrII-D group. Histochemical staining for reactive oxygen species indicated that phytoplasma infected (PI) tissues possess significantly higher levels of hydrogen peroxide (H2O2) rather than superoxide (O2 ·-) as compared with symptomless tissues. PI tissues were also associated with a significant increase in antioxidant enzyme activities (catalase, peroxidase, polyphenol oxidase, and glutathione reductase) and electrolyte leakage as compared with symptomless tissues. |
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