| 中国野生葡萄抗黑痘病基因的RAPD标记 |
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| 引用本文： | 张剑侠,王跃进,王西平,周鹏,郑学勤. 中国野生葡萄抗黑痘病基因的RAPD标记[J]. 果树学报, 2001, 18(2): 68-71 |
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| 作者姓名： | 张剑侠 王跃进 王西平 周鹏 郑学勤 |
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| 作者单位： | 1. 西北农林科技大学园艺学院,
2. National Key Biotechnology Laboratory for Tropical Crops,Chinese Academy of Tropical Agricultural Science, |
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| 基金项目： | 国家自然科学基金资助项目! (39770 5 2 5 )，高校博士点基金 !(980 70 1)，教育部资助年青优秀教师基金项目 |
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| 摘 要： | 应用随机扩增多态性DNA(RAPD)技术 ,对抗病×感病的葡萄种间杂交组合白河 35 1×佳利酿的亲本及其 2 3株F1代单株进行了抗黑痘病的遗传分析 ,通过对 196个随机引物的筛选获得了 1个与中国野生葡萄抗黑痘病基因连锁的RAPD标记OPS0 3 130 0 ,并在所有供试的中国野生葡萄 7个种的 2 0个株系和欧洲葡萄 8个品种中得到了验证。这一分子标记将为葡萄抗病育种和深入研究中国野生葡萄的抗黑痘病基因提供DNA依据
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| 关 键 词： | 中国野生葡萄 黑痘病 抗病基因 RAPD |
| 文章编号： | 1009-9980-(2001)-02-068-04 |
| 修稿时间： | 2000-06-25 |
RAPD Marker of Resistance Gene to Anthracnose in Chinese Wild Vitis |
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| Zhang Jianxia,Wang Yuejin,Wang Xiping,Zhou Peng,Zheng Xueqin. RAPD Marker of Resistance Gene to Anthracnose in Chinese Wild Vitis[J]. Journal of Fruit Science, 2001, 18(2): 68-71 |
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| Authors: | Zhang Jianxia Wang Yuejin Wang Xiping Zhou Peng Zheng Xueqin |
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| Abstract: | The genetic analysis of the resistance to Anthracnose (Spheceloma ampelinum de Bary.) of Chinese wild Vitis was carried out with the parents and 23 F1 individuals of interspecific hybridization combination Baihe-35-1×Carignane (Resistant×Susceptible) by Randomly Amplified Polymorphic DNA (RAPD) technique.A total 196 random primers were screened on the DNA of resistant and susceptible materials.OPS 03-1 300,a RAPD marker linked to Anthracnose resistant gene of Chinese wild Vitis,was obtained.The RAPD maker was varified in 20 clones of 7 Chinese wild Vitis species and 8 varieties of Vitis vinifera.Therefore,the DNA foundations were provided for the further assist breeding with the molecular marker of resistance gene and for the further research on Anthracnose resistant gene in Chinese wild Vitis. |
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| Keywords: | Chinese wild Vitis Anthracnose Resistance gene RAPD |
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