新疆苜蓿愈伤组织再生体系建立的研究

以新牧1号、新疆大叶苜蓿和新疆野生黄花苜蓿无菌苗下胚轴为外植体,研究了两种诱导培养基对愈伤组织诱导、生长的影响,以及增殖培养基中附加GSH和ABA对愈伤组织增殖、分化及再生的影响.结果表明,MS+2,4-D 2mg/L+NAA 2mg/L+KT 1mg/L为较佳诱导培养基;增殖培养基中附加GSH 15mg/L有利于分化及植株再生;增殖培养基中附加GSH 15mg/L+ABA 3 mg/L有利于提高新牧1号和新疆大叶苜蓿体胚分化能力,附加GSH 15mg/L+ABA 6mg/L有利于新疆野生黄花苜蓿体胚分化频率;实验共筛选出新牧1号、新疆大叶苜蓿、新疆野生黄花苜蓿高分化能力基因型分别为5、6和5个.

Study on Regeneration System of Alfalfa in Xinjiang

The paper studied effects of two kinds of induction medium on induction and growth of callus, using hypocotys of Medicago sativa L.cv. Xinjiang Daye 、Medicago varia.cv. Xinmu No.1 and Medicago Falcata.L aseptic seedlings as explants,and effects of GSH and ABA on enrichment, differentiation and regeneration callus.The results are as follows :①MS+2,4-D 2mg/L+NAA 2mg/L+KT 1mg/L was the better induction medium,②Adding GSH 15mg/L in enrichment medium is benefit to differentiation and regeneration ,③Adding GSH 15mg/L+ABA 3mg/L in enrichment medium had the best effects on Medicago sativa L.cv. Xinjiang Daye and Medicago varia.cv. Xinmu No.1, and GSH 15mg/L+ABA 6mg/L had the best effects on Medicago Falcata.L . ④The experiment separately selected 5、6、5 geneotypes with high differentiation capability for Medicago sativa L.cv. Xinjiang Daye、Medicago varia.cv. Xinmu No.1 and Medicago Falcata.L .