2011年云南烟草病毒病发生动态及复合侵染分析

 烟草病毒病发生普遍,在我国部分烟区已上升为烟草的第一大病害。烟草是云南省主要经济支柱之一,然而,病毒病的发生严重影响烟叶的产量和质量,造成一定经济损失。20世纪80～90年代,我国系统地调查了全国烟草侵染性病害,并对侵染我国烟草的病毒种类和严重影响生产的主要种类进行鉴定检测。2005年Zhang等^［1］对云南烟草病毒病及病原进行了研究。但是随着种植业结构的调整,品种的更换,烟草病毒种类出现新的变化,在田间病毒侵染情况也会发生变化。因此,有必要重新调查和鉴定云南烟草病毒种类,弄清影响云南烟草的病毒种类和优势种类,明确其复合侵染情况,从而指导病毒病的防控。

Analysis of dynamic occurrence and complex infection of tobacco viruses in 2011,Yunnan

The total 73 different tobacco virus disease samples collected from different regions in Yunnan were detected with electron microscopy, DAS-ELISA, immunostrip test and multiplex RT-PCR. The results showed that the tobacco virus diseases were serious in Yunnan Province. 10 kinds of viruses were detected in the experiments. The viruses were more at the northeast and central part, such as Chuxiong and Wenshan, than the north part of Yunnan. The results showed that main viruses were Tobacco mosaic virus(TMV), Cucumber mosaic virus (CMV), Potato virus Y(PVY), Tobacco etch virus (TEV) and Tobacco vein banding mosaic virus(TVBMV). The detection results showed there were nine types complex infections. The complex infection sample was 31.50% among the total testing samples. The main type of complex infection was that TEV and TVBMV, occupied 34.78% among the all combination.