黄曲霉毒素B1残留ELISA试剂盒的研制及应用

本研究采用间接竞争酶联免疫吸附试验(ELISA)检测黄曲霉毒素B₁(aflatoxins B₁,AFB₁)残留量。将AFB₁与蛋白质载体牛血清蛋白(BSA)和卵清白蛋白(OVA)偶联制备了AFB₁-BSA和AFB₁-OVA偶联物,以AFB₁-BSA作为免疫原制备特异性抗体,并成功建立了AFB₁残留间接竞争ELISA检测方法及检测试剂盒。试剂盒IC₅₀为128.8 ng/L,检测线性范围为50~1800 ng/L,检测限不超过100 ng/kg;食用油、花生和谷物的平均添加回收率大于71.3%,批内、批间变异系数均小于14.2%。因此,本试剂盒具有操作简便、检测快速、灵敏度高等特点,将在植物性食品中AFB₁的残留检测中发挥重要作用。

Study and Application on the ELISA Kit for Detecting Aflatoxins B1

This study was to aim to use ELISA method for rapid screening of the aflatoxins B₁(AFB₁) residues. Coupled AFB₁ to bull serum albumin(BSA) and ovalbumin(OVA) to obtain AFB₁-BSA and AFB₁-OVA respectively. Specific antibody against AFB₁ was prepared with AFB₁-BSA and ELISA kit,and had been successfully developed for detecting AFB₁ residues. The IC₅₀ of the kit was 128.8 ng/L,linear detection range was 50~1800 ng/L with the lowest detection limit less than 100 ng/kg. The recovery of AFB₁ in edible oil,peanut and cereal was more than 71.3%. Coefficient of variation for repeatability tests was less than 14.2%.Therefore,the kit was simple,fast,sensitive,and would be play an important role in AFB₁ residues detection in the plant food.