紫花槭秋季叶片呈色生理变化研究

为了探寻影响紫花槭叶色变化的主要因素,从而为其园林配置提供理论依据,对紫花槭秋季叶片转色不同时期的叶色参数、色素含量、相关酶活性、碳水化合物和矿质元素含量进行了测定。结果表明:1)紫花槭叶色参数L值先上升后降低,a值呈持续上升趋势,b值呈先上升后下降再上升的趋势;2)紫花槭叶片叶绿素含量呈下降趋势,类胡萝卜素含量呈先下降后上升再下降的趋势,花色素苷含量呈上升趋势;3)苯丙氨酸解氨酶(PAL)、查尔酮异构酶(CHI)、查尔酮还原酶(CHR)活性均呈先升高后降低的趋势;4)碳水化合物中蔗糖含量呈先下降后上升再下降的趋势;5)矿质元素中氮、钾含量的变化趋势相同,均呈下降趋势,磷含量呈先上升后下降的双峰趋势,镁含量呈先上升后下降的趋势,钙含量呈先下降后上升再下降的趋势。对不同色素与其他生理指标的相关性分析结果表明:叶绿素含量与氮、钾均存在极显著的正相关关系,与钙存在显著的正相关关系;类胡萝卜素含量与其他指标的相关性均未达到显著水平;花色素苷的含量与PAL、CHI酶活性和氮、钾含量间均呈极显著负相关,与CHR酶活性及钙、镁含量间均呈显著负相关,而与淀粉、蔗糖含量间均存在正相关,但不显著;色素与磷含量无明显的相关性。

Study on physiological change of Acer pseudo-sieboldianum leaf color in autumn

In order to research main factors affecting change of Acer pseudo-sieboldianum leaf color and to provide some theoretical bases for garden configuration,leaf color parameters,pigment content,related enzyme activity,carbohydrate content and mineral element content in A.pseudo-sieboldianum leaves were measured during leaf color changing. The results showed that,L value of leaf color parameter was firstly increased and then decreased,a value showed a continuous upward trend,and b value was firstly increased,decreased and then increased.Chlorophyll content in A. pseudo-sieboldianum leaves showed a downtrend,carotenoid content was firstly decreased,increased and then decreased, and anthocyanin content was on the rise.Activities of phenylalanine ammonia lyase (PAL),chalcone isomerase (CHI), chalcone reductase (CHR) were firstly increased and then decreased.Sucrose content in carbohydrates was firstly decreased,increased and then decreased.Changes of both N and K contents in mineral elements showed the same downtrend.P content showed a bimodal trend of rising firstly and then going down.Mg content was firstly increased and then decreased.Ca content showed a trend of firstly going down,rising and then going down.The results of correlation analysis between different pigments and other physiological indexes showed that chlorophyll content had significant positive correlations with N content and K content,and a significant positive correlation with Ca content.Carotenoid content had no significant correlation with other indexes.Anthocyanin content was very significantly negatively correlated with PAL activity,CHI activity,N content and K content,and significantly negatively correlated with CHR activity,Ca content and Mg content.Anthocyanin content was positively correlated with starch content and sucrose content,but not significant.There was no obvious correlation between pigment content and P content.