苹果黑星病菌的早期分子检测

苹果黑星病(Venturia inaequalis)是中国苹果产区的重要病害之一,为了建立该病原菌的早期快速检测技术,筛选出了对苹果黑星病菌具有特异性的PCR引物.利用这对引物能从苹果黑星病菌基因组DNA中扩增出一条分子量为190 bp的特异性条带,准确地把苹果斑点落叶病、苹果褐斑病和苹果白粉病等常发性苹果叶斑病害区分开.采用改进的CTAB法,快速提取发病叶片组织的DNA,并结合PCR检测技术,可从苹果黑星病显症叶片以及未显症而处于潜育期的叶片组织中特异性地检测到苹果黑星病菌.结果表明,建立的苹果黑星病菌分子检测方法,可用于该病害的早期快速分子诊断.

Early Molecular Detection of Venturia inaequalis of Apple

Apple scab (Venturia inaequalis) is one of most important apple diseases in apple growing areas in China. Through screening a pair of specific PCR primers for pathogen of apple scab, the method of early and rapid detection of pathogen of apple scab was developed in the study. Based on u-sing those specific PCR primers, a unique 190 bp band was obtained by PCR amplification from ge-nomic DNA of Venturia inaequalis of apple and it can be accurately distinguished from pathogens of Alteraria mali, Marssonia mali and Podosphaera leucotricha , the common apple leaf spot diseases. The total genomic DNA of diseased apple leaves was rapidly obtained by modified CTAB method. Through PCR amplification assay, the pathogen of apple scab can be specifically detected from diseased apple leaf tissues and no symptom appearance of apple leaf tissues with pathogen incubation. The PCR based method developed here could be used for early and rapid molecular detection of pathogen of apple scab.