苏云金杆菌杀虫晶体蛋白基因转化美洲黑杨的研究

本研究首先建立了美洲黑杨叶片外植体的再生系统，并利用Ｈｏｒｓｃｈ等人１９８５年发明的叶盘法，将分别带有嵌合基因ＮＰＴＩＩ和１．８ｋｂ或２．１ｋｂ Ｂｔ．基因的土壤杆菌ＬＢＡ４４０４与美洲黑杨叶片共培养。采用３０ｍｇ／ｌ和５０ｍｇ／ｌ两种浓度的卡那霉素筛选转子，约２８天左右的时间，有不定芽从外植体切口处形成。两种卡那霉素浓度下共形成２７５个伸长芽，存活了１８７株。这１８７株分别进入卡那霉素３０ｍｇ／

STUDY ON THE PLANT REGENERATION FROM POPULUS DELTOIDES EXPLANT TRANSFORMED WITH BT. TOXIN GENE

The effective regenetation system for Populus deltoides was developedfirstly in the experiment.Based on the system,Agrobacterium tumefaciensLBA 4404 strains hauboring the Bt.toxin gene expression vectors pB48.214and pB48.215 respectively were uesd for transformation of poplar plants bymeans of the leaf disc method.After transformating,adventitious buds wereformed at the cut of explant on the media with two concentrations of kanamycin 50mg/l and 30mg/l nearly one month later.Variance analysis showedthat the effects of two kanamycin concentrations on the transformation rateand mean adventitious bud numbers were significant at p<0.05.When shootsreached 1cm long,they were transferred to rooting media and supplementedwith 30mg/l and 50mg/l kanamycin and the rooting percentage was 9.62%and 8.02% respectively.PCR analysis showed that Bt.genes were successfully transformed into Populus deltoides chromosomes.