| 核桃茎尖培养中防止褐变的方法 |
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| 引用本文： | 张俊林,刘庆忠,刘松,樊靖,高扬,秦岭.核桃茎尖培养中防止褐变的方法[J].北京农学院学报,2007,22(2):17-19. |
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| 作者姓名： | 张俊林 刘庆忠 刘松 樊靖 高扬 秦岭 |
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| 作者单位： | 北京农学院,植物科学技术系,北京,102206;山东省果树研究所,山东,泰安,271000;山东省果树研究所,山东,泰安,271000;北京市园林绿化局,北京,100029;西南林学院,云南,昆明,650224;北京农学院,植物科学技术系,北京,102206 |
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| 基金项目： | 国家科技部基础工作资助项目
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北京市教委都市农业学科群资助项目 |
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| 摘 要： | 针对核桃茎尖初代培养易褐变的问题,以3个核桃品种为试材,研究了预处理和培养基中添加抗氧化剂和吸附剂对褐变程度的影响。结果表明:经1～2℃冷藏处理24 h,20%的Na2S2O3浸泡20～30 min,流水冲洗8～10 h并在培养基中添加2.0 g/L PVP的处理,在短期内可有效地防止3个品种的褐变。
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| 关 键 词： | 核桃 茎尖 离体培养 褐变 品种 |
| 文章编号： | 1002-3186（2007）02-0017-03 |
| 修稿时间： | 2007-01-25 |
Methods of Browning Control in Walnut Shoot Tips Culture |
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| ZHANG Jun-lin,LIU Qing-zhong,LIU Song,FAN Jing,GAO Yang,QIN Ling.Methods of Browning Control in Walnut Shoot Tips Culture[J].Journal of Beijing Agricultural College,2007,22(2):17-19. |
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| Authors: | ZHANG Jun-lin LIU Qing-zhong LIU Song FAN Jing GAO Yang QIN Ling |
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| Abstract: | Three walnut cultivars were used as experiment materials,different methods was used to pretreatment of walnut explants and added antioxidants or adsorbents to primary medium to study on the walnut shoot tips browning.The result showed that : the effective method to anti-browning for three cultivars in short-term was that shoot tips of walnut was pretreatment under cold storage at 1~2 ℃ for 24 h;then dipped in the 20%sodium thiosulfatef for 20~30 minutes and subsequently washed with tap water for 8~10 hours,and then,cultured in the medium added with PVP 2.0 g/L. |
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| Keywords: | Juglans regia shoot tips in vitro culture browning cultivar |
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