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| 鸡贫血病毒VP1基因的克隆及其在大肠杆菌中的表达 | |
| 引用本文： | 黄冬艳,杨兵,齐欣,陈小玲,徐福洲.鸡贫血病毒VP1基因的克隆及其在大肠杆菌中的表达[J].华北农学报,2006,21(5):50-53. |
| 作者姓名： | 黄冬艳 杨兵 齐欣 陈小玲 徐福洲 |
| 作者单位： | 1. 江西农业大学,动物科学院,江西,南昌,330045 2. 北京市农林科学院畜牧兽医研究所,北京,100089 3. 沈阳农业大学,畜牧兽医学院,辽宁,沈阳,110161 |
| 摘 要： | 根据鸡贫血病毒Cux_1株基因组序列设计了一对引物,通过PCR扩增出VP1基因。将扩增出的片段克隆到pGM_T载体上,通过测序分析证实该片段与鸡贫血病毒Cux_1株的VP1基因序列一致。然后将克隆化VP1基因亚克隆到PET_32a( )载体上并进行原核表达。SDS_PAGE和Western Blot分析表明,一个分子质量为70.4 kDa的重组蛋白获得了成功表达,并且鸡贫血病毒阳性血清能和重组蛋白发生反应。 |
| 关 键 词： | 鸡贫血病毒 VP1基因 克隆 表达 |
| 文章编号： | 1000-7091（2006）05-0050-04 |
| 收稿时间： | 2006-03-12 |
| 修稿时间： | 2006-03-12 |
Cloning of VP1 Gene of Chicken Infectious Anemia Virus and Its Expression in E. coli |
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| HUANG Dong-yan,YANG Bing,QI Xin,CHEN Xiao-ling,XU Fu-zhou.Cloning of VP1 Gene of Chicken Infectious Anemia Virus and Its Expression in E. coli[J].Acta Agriculturae Boreali-Sinica,2006,21(5):50-53. | |
| Authors: | HUANG Dong-yan YANG Bing QI Xin CHEN Xiao-ling XU Fu-zhou |
| Institution: | 1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; 2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China;3. College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, China |
| Abstract: | We designed a pair of oligonucleotide primers based on the genomic sequence of CAV Cux-1 strain,and amplified VP1 gene by PCR.The amplification fragment was cloned into pGM-T easy vector.The result of nucleotide sequence analysis demonstrated that the cloned genomic DNA was identical with that deduced from Cux-1 strain of CAV.Then the cloned genomic DNA was subcloned into prokaryotic expression vector PET-32a( ),and the recombinant protein was expressed in E.coli cell.SDS-PAGE and Western Blot analysis showed that a protein with a molecular weight of 70.4 kDa was successfully expressed and CAV-infected chicken serum reacted with the recombinant protein. |
| Keywords: | Chicken infectious anemia virus VP1 Cloning Expression E coli |
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