中国野生鸭茅遗传多样性的ISSR研究

以宝兴鸭茅和国外安巴鸭茅品种为对照,用ISSR分子标记技术对四川、重庆、云南、贵州、新疆等地的32份野生鸭茅材料进行遗传多样性研究。试验筛选出引物12个,共扩增出多态性带88条,平均每个引物扩增的多态带数为7.3条,多态性条带比率(PPB)为83.18%,材料间遗传相似系数为0.692 0~0.926 0。这说明我国鸭茅具有较丰富的遗传多样性。根据研究结果进行了聚类分析和主成分分析,可将32份中国野生鸭茅材料分为五大类,同一地区的鸭茅品种(系)基本聚在同一类,呈现出一定的地域性分布规律。并对我国鸭茅种质资源的收集保存提出建议。

Genetic diversity of wild Dactylis glomerata in China detected by inter-simple sequence repeat (ISSR) molecular markers

In a comparison of Dactylis glomerata cv.Baoxing with D.glomerata cv.Amba,Inter-simple Sequence Repeat(ISSR) molecular markers were used to detect the genetic diversity among 30 specimens of D.glomerata.Collected from Sichuan,Chongqing,Yunnan,Guizhou,and Xinjiang provinces.The twelve primers selected for use and produced a total of eighty-eight polymorphic bands,an average of 7.3 bands from each pair of ISSR primers.The average percentage of polymorphic bands was 83.8%,and the range of GS was(0.692 0)-0.926 0,which indicated a rich genetic diversity of D.glomerata in China.Based on cluster and principal component analyses of the genetic characteristics,the wild D.glomerata could be divided into 5 groups according to the nearest phylogenetic relationship.In most cases materials from the same region were in the same group thus showing a geographical distribution of genetic diversity of the tested materials.Advice is given on the collection and conservation of germplasm resources in wild D.glomerata populations.