棉花黄萎病拮抗细菌2-70（Bacillus subtilis）菌株定殖能力的研究

试验通过灭菌土和非灭菌土两种土样，试验前分别对这两种土样进行三种不同的处理，即：用拮抗菌芽孢液浸种，土壤全部混菌，部分混菌进行盆栽试验。主要通过盆栽试验，考察棉花黄萎病拮抗细菌2—70在土壤中的定植情况及在棉花根内和根际的定植情况。结果表明，在实验室条件下，拮抗细菌2．70能够在灭菌土和非灭菌土中定殖，且定殖数量达10^6cfu／g土左右，经过五个月后仍能保持较高的抑菌活性，并且菌株在灭菌土中的定殖数量高于非灭菌土中的数量。通过对棉苗根内细菌的回收，证实拮抗细菌2．70能在棉花根内定殖，数量达到10^3cfu／g。

Studies on colonization of Antagonistic Bacteria 2-70(Bacillus subtilis) to Cotton Verticillium dahliae

This test required sterile soil and non-sterile soil in two soil samples, test separately for the two soil samples of three different treatments that is: The antagonistic bacterium Bacillus soaking liquid, soil all mixed soil and some soil for potted plants mixed. In this paper, a pot experiment to study Verticillium dahliae 2-70 antagonistic bacteria in the soil and in the colonization of cotton roots and rhizosphere colonization of the situation. The results showed that under laboratory conditions, antagonistic bacteria can 2-70 in non-sterilized soil and sterilized soil colonization, and colonization Tatsu quantity 106 cfu/g soil around, after five months has managed to maintain a higher antibacterial activity, and strains in sterile soil in the quantity colonization than non-sterilized soil in quantity. Cotton roots by bacteria recovery, confirmed the antagonistic bacteria in the 2-70 designated cotton root colonization, number of 103cfu/g.