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| 猪繁殖与呼吸综合征病毒双基因多组合DNA疫苗的构建 | |
| 引用本文： | 赵永刚,薛强,周艳君,仇华吉,萨仁高娃,童光志,申之义. 猪繁殖与呼吸综合征病毒双基因多组合DNA疫苗的构建[J]. 中国动物检疫, 2009, 26(5): 33-37 |
| 作者姓名： | 赵永刚 薛强 周艳君 仇华吉 萨仁高娃 童光志 申之义 |
| 作者单位： | 1. 中国动物卫生与流行病学中心,266032;中国农业科学院哈尔滨兽医研究所,150001 2. 中国检验检疫科学研究院,100025 3. 中国农业科学院哈尔滨兽医研究所,150001 4. 中国科学院海洋研究所,266003 5. 内蒙古农业大学,010018 |
| 摘 要： | 本实验根据PRRSV结构蛋白的特性和IFNγ及IL-2这两种细胞因子的特性,构建表达PRRSVGP4-GP5、GP5-M、GP5-N、GP5-IFNγ、GP5-IL-2、N-IFNγ、N-IL-2的重组质粒。这些重组质粒在真核细胞中得到了表达。用这些真核表达质粒可以免疫小猪,通过检测其对猪体免疫应答的诱导能力和接种后动物对PRRSV的抵抗力来对基因疫苗的效能进行研究,以期筛选到有效的基因疫苗并为今后进一步的研究提供思路和理论基础。 |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 基因免疫 重组质粒 |
Construction of Double Gene and Multi-Combination DNA Vaccines for PRRSV |
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| Zhao Yonggan,Xue Qiang,Zhou Yanjun,Chou Huaji,Saren Gaow,Tong Guangzhi,Shen Zhiyi. Construction of Double Gene and Multi-Combination DNA Vaccines for PRRSV[J]. China Animal Health Inspection, 2009, 26(5): 33-37 | |
| Authors: | Zhao Yonggan Xue Qiang Zhou Yanjun Chou Huaji Saren Gaow Tong Guangzhi Shen Zhiyi |
| Affiliation: | Zhao Yong-gan1,2,Xue Qiang4,Zhou Yan-jun2,Chou Hua-ji2,Saren Gaowa5,Tong Guang-zhi2,Seng Zhi-yi3 (1.China Animal Health&Epidemiology Center,Qingdao, 266032; 2.Harbin Veterinary Resenrch Institute,CAAS, 150001; 3.Inner Mongolia Agricultural University,Huhhot 010018; 4.Chiueea Academy of inspection and Quaranline Seience,Beijing 100025; 5.Oceanology Institnce,Chinese Academy of Sciences,Qingdao 266032) |
| Abstract: | According to the features of PRRSV structure proteins and the two cell factors:IFN-r and IL-2,re-combinant plasmids PRRSV GP4-GP5,GP5-M,GP5-N,GP5-IFNr,GP5-IL,N-IFNr,N-IL2 were constructed and expressed in eukaryotic cells.The expressed plasmids were used to immunize piglets.The immcne efficiency was studied by detecting their immune response-inducing ability in pigs,and resistance of the immunized animals to PRRSV infection,in an attempt to screen gene vaccines and provide new thinking and theoretical base for funther research. |
| Keywords: | Prrsv Gene vaccine Recombinant plasmid |
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