牛乳腺上皮细胞β-酪蛋白的检测及核型分析

本试验用Western-blotting检测奶牛乳腺上皮细胞培养液中的β 酪蛋白，鉴定该细胞的生理功能是否正常；通过分析染色体数目及核型以鉴定体外培养的奶牛乳腺上皮细胞是否发生转化。结果显示：培养液中含有β 酪蛋白，说明该乳腺上皮细胞生理状况良好；染色体数目正常，染色体数目为60，核型与哺乳动物染色体图谱一致，说明该细胞系在体外培养条件下未发生转化。

Bovine Mammary Epithelial Cells beta-Casein Protein Detection and Karyotype Analysis

Using the Western-blotting technique to detect the beta-casein protein from culture medium of bovine mammary epithelial cells(BMEC) in vitro,which can identify the normal physiological function of BMEC;Karyotype analysis identify the number and figure of Chromosome of BMEC cultured in vitro.The results show: containing beta-casein protein in culture medium of bovine mammary epithelial cells in vitro shows that the mammary epithelial cells with normal physiological condition;the number of chromosomes in normal,it is 60, and the karyotype agreement with the mammalian chromosome map,shows that the cell lines in vitro culture conditions not transformation.