| 适于TAIL-CR模板的水稻基因组DNA提取方法的优化 |
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| 引用本文： | 袁云香,孙丙耀. 适于TAIL-CR模板的水稻基因组DNA提取方法的优化[J]. 安徽农业科学, 2009, 37(9): 3964-3966 |
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| 作者姓名： | 袁云香 孙丙耀 |
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| 作者单位： | 渭南师范学院环境与生命科学系,陕西渭南,714000;苏州大学基础医学与生物科学学院,江苏苏州,215006 |
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| 基金项目： | 国家自然科学基金项目资助(30571049); 渭南师范学院研究生科研基金项目资助(09YKZ002) |
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| 摘 要： | [目的]筛选适合作为TAIL-PCR模板的水稻基因组DNA提取方法。[方法]采用5种方法提取水稻叶片基因组DNA,筛选出DNA纯度及浓度较高的、适合作为TAIL-PCR模板的水稻基因组DNA提取方法。[结果]改良的SDS法及CTAB法提取的基因组DNA纯度及浓度较高,效果明显优于脲法及简易法。以TAIL-PCR技术对选择的同一个含Ds元件的样品的5种提取方法的基因组DNA作为模板进行Ds侧翼序列的扩增,改良的SDS法及CTAB法得到的条带特异,这些产物经回收、纯化后可直接用于序列的测定。但考虑到CTAB价格比较昂贵,毒性较大,一般不主张采用。[结论]改良的SDS法提取的水稻基因组DNA最适作为TAIL-PCR的模板。
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| 关 键 词： | 水稻 基因组DNA 提取方法 |
Optimization of the Extraction Method of Rice Genomic DNA Suitable for TAIL-PCR Template |
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| YUAN Yun-xiang et al. Optimization of the Extraction Method of Rice Genomic DNA Suitable for TAIL-PCR Template[J]. Journal of Anhui Agricultural Sciences, 2009, 37(9): 3964-3966 |
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| Authors: | YUAN Yun-xiang et al |
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| Affiliation: | YUAN Yun-xiang et al (Department of Environment , Life Sciences,Weinan Normal University,Weinan,Shaanxi 714000) |
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| Abstract: | [Objective] The aim was to screen the extraction method of rice genomic DNA suitable for taking as TAIL-PCR template.[Method] The genomic DNA of rice leaf were extracted by 5 kinds of method,and the extraction method of rice genomic DNA suitable for taking as TAIL-PCR template was screened and the purity and concn.of the DNA extracted by the method were higher.[Result] The purity and concn.of the genomic DNA extracted by the method of improved SDS and CTAB were higher and the effects were better than that o... |
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| Keywords: | Rice Genomic DNA Extraction method |
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