山苍子外植体初始诱导的研究

[目的]研究山苍子外植体的诱导方法。[方法]以不同类型、不同采集时间的山苍子茎段为外植体材料进行组织培养研究。[结果]以0.1%氯化汞对外植体处理10 min效果较好,此时外植体污染率为40.0%,死亡率为15.0%;以顶芽以下2～5个芽的茎段进行初始诱导效果较好;1月份为最佳取材时间,此时诱导率高达81.1%,污染率仅为11.7%;初始诱导中,以改良MS+1.0 mg/L 6-BA+0.2mg/L NAA培养基为最适培养基配方。[结论]1月采集的半木质化茎段用0.1%氯化汞消毒10 min后,以改良MS+1.0 mg/L 6-BA+0.2 mg/L NAA培养基可较好诱导山苍子幼苗。

Research on Initial Explants Induction of Litsea cubeba

[Objective] The aim of this study was to explore an explants induction method for L.cubeba.[Method] Different types of L.cubeba stem segments were collected at different time as explants materials for induction and culture.[Result] The results showed that sterilization with 0.1% HgCl2 for 10 min achieved better effects,with an explants contamination rate of 40.0% and a mortality rate of 15.0%;stem segments of the 2-5 buds below terminal bud were better explants materials for initial induction;the best sampling time was January,with an induction rate as high as 81.1%,while the contamination rate was only 11.7%;modified MS+1.0 mg/L of 6-BA+0.2 mg/L of NAA was the optimal medium for explants induction of L.cubeba.[Conclusion] Semi-lignified stem segments of L.cubeba collected in January,sterilized with 0.1% HgCl2 for 10 min and cultured in modified MS+1.0 mg/L of 6-BA+0.2 mg/L of NAA had achieved better induction effects on L.cubeba seedlings.