| 中药萝藦中C21甾苷的含量测定 |
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| 引用本文： | 杨蕾,陈强,宋洁琼,张文清.中药萝藦中C21甾苷的含量测定[J].安徽农业科学,2012,40(2):738-739. |
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| 作者姓名： | 杨蕾 陈强 宋洁琼 张文清 |
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| 作者单位： | 上海市功能性材料化学重点试验室,华东理工大学,上海200237;上海市功能性材料化学重点试验室,华东理工大学,上海200237;上海市功能性材料化学重点试验室,华东理工大学,上海200237;上海市功能性材料化学重点试验室,华东理工大学,上海200237 |
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| 摘 要： | 目的]测定萝藦中C21甾苷的含量。方法]以孕甾烯醇酮为标准物,采用磺基醋酸汞试剂法于510 nm波长处测定C21甾苷的吸光度值,并计算其含量。结果]萝藦中C21甾苷含量为0.003 3 mg/ml,与相关文献中萝藦科植物中甾苷的含量接近。结论]该方法准确率、重现性、线性关系等都满足科研和生产要求,可以用于萝藦中C21甾苷的含量测定。
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| 关 键 词： | 萝藦 C21甾苷 磺基醋酸汞试剂法 含量 |
The Determination on the Content of C21 Steroidal Glycosides in Metaplexis japonica ( Thunb.) Makino |
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| Institution: | YANG Lei et al (Shanghai Key Laboratory of Functional Materials Chemistry,East China University of Science and Technology,Shanghai 200237) |
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| Abstract: | Objective] The aim was to determine the content of C21 steroidal glycosides in M.japonica.Method] With pregnenolone as reference substance,sulfonic mercuric acetate reagent method was used to determine the Absorbance of C21 steroidal glycosides at 510 nm and calculate it′s content.Result] The content of C21 steroidal glycosides in M.japonica was 0.003 3 mg/ml and was similar to the content of Asclepiadaceae plants in relevant documents.Conclusion] The accuracy rate,repeatability and linear relation of this method were met the scientific research and production requirements,thus,can be used to determine the content of C21 steroidal glycosides in M.japonica. |
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| Keywords: | Metaplexis japonica(Thunb )Makino C21 steroidal glycosides Sulfonic mercuric acetate reagent Content |
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