| 柳叶中黄酮醇苷元槲皮素的含量测定 |
| |
| 引用本文： | 刘卫,邓淑凤,刘景东,秦葵,孙晓丽.柳叶中黄酮醇苷元槲皮素的含量测定[J].安徽农业科学,2012,40(2):761-762. |
| |
| 作者姓名： | 刘卫 邓淑凤 刘景东 秦葵 孙晓丽 |
| |
| 作者单位： | 第四军医大学白求恩军医学院,河北石家庄,050081;第四军医大学白求恩军医学院,河北石家庄,050081;第四军医大学白求恩军医学院,河北石家庄,050081;第四军医大学白求恩军医学院,河北石家庄,050081;第四军医大学白求恩军医学院,河北石家庄,050081 |
| |
| 摘 要： | 目的]测定柳叶中黄酮醇苷元成分槲皮素的含量。方法]于4月中旬采摘柳叶嫩叶,用无水甲醇连续提取,将提取液用盐酸水解后采用高效液相色谱法测定槲皮素的含量。色谱条件为:色谱柱为Diamonsil C18柱(150 mm×4.6 mm,5μm),流动相为浓度0.07%磷酸-无水甲醇(50∶50,V/V);流速为1.0 ml/min;检测波长为260 nm;进样量为10μl;灵敏度为0.000 1 AUFS;柱温为室温(20℃)。结果]柳叶嫩叶中黄酮醇苷元以槲皮素为主,含量在0.36%～0.74%。结论]柳叶中槲皮素含量测定方法的建立,为有效利用现有柳资源提供理论依据。
|
| 关 键 词： | 柳叶 槲皮素 高效液相测定 |
Determination of Quercitin in Willow Leaf |
| |
| Institution: | LIU Wei et al(Bethune Military Medical College of the Forth Military Medical University,Shijiazhuang,Hebei 050081) |
| |
| Abstract: | Objective] The aim was to develop a method of RP-HPLC for determination of flavnoid aglycones―quercitin in willow leaf,and to discover its content.Method] Willow leaves were collected in the middle of April.The flavonoids in the leaves were extracted by successive extraction with methanol.The extracts were acid-hydrolyzed before being tested with RP-HPLC.The condition of RP-HPLC was:Diamonsil C18 column(150 mm×4.6 mm,5 μm);concentration of mobile phase was 0.07%;phosphoric acid-water-free ethanol(50:50,V/V);mobile speed was 1.0 ml/min;tested wave length 260 nm;sampling amount 10 μl;flexibility 0.000 1 AUFS;column temperature was indoor temperature(20 ℃).Result] The constituent of flavnoid aglycones in willow leaves was mainly quercitin and it consisted of 0.36%-0.74% flavnoid aglycones.Conclusion] The method of RP-HPLC for determination of quercitin in willow leaf provided reference for the effective use of willow. |
| |
| Keywords: | Quercitin Willow leaf RP-HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
