银杏叶中黄酮类化合物的提取工艺研究

目的]研究银杏叶中黄酮类化合物的超声提取工艺。方法]采用超声法进行提取,然后用紫外可见分光光度法进行测定,考察了提取银杏叶中黄酮类化合物的影响因素,优选最佳超声提取条件。结果]超声波辅助提取银杏叶中黄酮的最佳条件为:浓度70%的乙醇为溶剂,pH值为5.0的酸性条件,提取时间为90 min,提取温度为60℃,振荡时间为10 min,振荡次数为3次;在此条件下,银杏叶中黄酮化合物的提取率为0.201 2%。结论]优选出了银杏叶中黄酮类化合物的最佳超声提取条件,为银杏叶的开发和利用提供了理论依据。

Study on the Extraction Technology of Flavonoids from G.biloba Leaves

Objective] To study the ultrasonic extraction technology of flavonoids from G.biloba leaves.Method] The flavonoids were first extracted from G.biloba leaves by ultrasonic method in different conditions then identified by UV spectrophotometry.On the basis of single-factor tests,the factors that affected the extraction of flavonoids were optimized.Result] The optimal parameters for extracting flavonoids from G.biloba leaves were based on the extraction for 90 min at 60 ℃ with 70% ethanol as solvent in the acidic condition(pH=5).Moreover,the extraction system oscillated for 3 times of 10 min each time.Under the above conditions,the yield of flavonoids was 0.201 2%.Result] This study screens out the optimum ultrasonic extraction technology of flavonoids,which could provide theoretical basis for the development and utilization of G.biloba leaves.