| 宜兴百合组培快繁技术的研究 |
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| 引用本文： | 潘理云,张海洋.宜兴百合组培快繁技术的研究[J].安徽农业科学,2012,40(10):5748-5750. |
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| 作者姓名： | 潘理云 张海洋 |
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| 作者单位： | 湖州师范学院生命科学学院,浙江湖州,313000;湖州师范学院生命科学学院,浙江湖州,313000 |
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| 基金项目： | 2011年浙江省大学生科技创新活动计划(新苗人才计划)资助项目(2011R425003) |
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| 摘 要： | 目的]采用组织培养的方法对宜兴百合(Lilium lancifoium Thumb)进行扩大繁殖研究。方法]以宜兴百合鳞茎为外植体,在其不同的组织培养阶段采用不同的激素配比,观察培养效果,以建立宜兴百合的组培快繁体系。结果]宜兴百合鳞茎不定芽诱导的最佳培养基为MS+3.0 mg/L 6-BA+0.5 mg/L NAA,继代培养的最佳培养基为MS+3.0 mg/L 6-BA+0.1 mg/L NAA,生根培养的最佳培养基为1/2MS+0.5 mg/L IBA。结论]为提高宜兴百合的繁殖系数和加快其产业化生产提供技术支撑。
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| 关 键 词： | 宜兴百合 组培 快繁 激素 |
Study on Tissue Culture Technology of Lilium lancifolium Thunb |
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| Institution: | PAN Li-yun et al(Huzhou Teachers College,Huzhou,Zhejiang 313000) |
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| Abstract: | Objective] The aim was to use tissue culture method for the increased reproduction of Lilium lancifoium Thumb.Method] Using bulbs of Lilium lancifoium Thumb as explants,observing the culture effect in its different tissue culture stages with different hormone combinations,to build Lilium lancifoium Thumb tissue culture system.Result] The best adventitious bud induction medium of Lilium lancifoium Thumb bulbs was MS+3.0 mg/L 6-BA+0.5 mg/L NAA,the best subculture medium was MS+3.0 mg/L 6-BA+0.1 mg/L NAA,the best root induction medium was 1/2MS+0.5 mg/L IBA.Conclusion] It provided technical support for improving the propagation coefficient of Lilium lancifoium Thumb and speeding up its industrial production. |
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| Keywords: | Lilium lancifoium Thumb Tissue culture Propagation Hormone |
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